{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:25:00Z","timestamp":1787765100626,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>T helper (Th) cells are involved in the pathogenesis of pemphigus vulgaris (PV). However, the mechanism still needs more exploration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>This study aimed to evaluate the molecular mechanism of the dysregulation of Th17 cells in the peripheral blood of patients with PV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Serum levels of IL-17 and anti-Dsg3 titres in patients with PV were analysed using ELISA. The mRNA expression of retinoic acid orphan receptor \u03b3t (ROR\u03b3t) in CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells was detected using reverse transcription\u2013quantitative PCR (qPCR). The number of Th17 cells was examined using flow cytometry. Western blot analysis and immunofluorescent staining were also performed to investigate the expression levels of ERK\/MAPK signalling proteins and Th17 lineage-associated proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The proportion of Th17 cells and Th17 spectrum-associated proteins (p-STAT3, ROR\u03b3t and IL-17) were upregulated in CD4+ cells in PV patients. The increased transcriptional levels of ROR\u03b3t and IL-17 correlated positively with the severity of PV. Elevated phosphorylation of the ERK signalling factors was found in the collected CD4+ T cells in PV patients. The inhibition of the ERK signalling pathway significantly reduced the differentiation of Th17 cells in PV patients\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Th17 cells are essential in the dysregulation of PV, and ERK signalling is involved in Th17-type immunity and promotes the development of PV. The study here provides us with a potential therapeutic target for PV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijd.ijd_924_22","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T05:06:38Z","timestamp":1704776798000},"page":"724-724","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["ERK\/MEK Pathway Regulates Th17 Cell Differentiation in Patients with Pemphigus Vulgaris"],"prefix":"10.4103","volume":"68","author":[{"given":"Kai","family":"Han","sequence":"first","affiliation":[{"name":"From the Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song-Shan","family":"Li","sequence":"additional","affiliation":[{"name":"From the Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Pan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, Rhode Island, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Nian","family":"Xu","sequence":"additional","affiliation":[{"name":"From the Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Zhen","family":"Zhong","sequence":"additional","affiliation":[{"name":"From the Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Dermatology, Nanfang Hospital, Southern 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