{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:42:57Z","timestamp":1770885777386,"version":"3.50.1"},"reference-count":18,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T00:00:00Z","timestamp":1612483200000},"content-version":"vor","delay-in-days":219,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Indian Journal of Medical Microbiology"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.4103\/ijmm.ijmm_20_249","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T04:09:00Z","timestamp":1604462940000},"page":"288-292","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Utility of a Multiplex Real-Time Polymerase Chain Reaction for Combined Detection and Serotyping of Dengue Virus in Paediatric Patients Hospitalised with Severe Dengue: A Report from Chennai"],"prefix":"10.1016","volume":"38","author":[{"given":"S.","family":"Balasubramanian","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Chandy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robinson","family":"Peter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gothai S.","family":"Nachiyar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amullya","family":"Sudhakar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sumanth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Manoharan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.4103\/ijmm.IJMM_20_249_bib1","doi-asserted-by":"crossref","first-page":"e1760","DOI":"10.1371\/journal.pntd.0001760","article-title":"Refining the global spatial limits of dengue virus transmission by evidence-based consensus","volume":"6","author":"Brady","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"10.4103\/ijmm.IJMM_20_249_bib2","doi-asserted-by":"crossref","first-page":"e0192672","DOI":"10.1371\/journal.pone.0192672","article-title":"Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season","volume":"13","author":"Shrivastava","year":"2018","journal-title":"PLoS One"},{"key":"10.4103\/ijmm.IJMM_20_249_bib3","doi-asserted-by":"crossref","first-page":"27663","DOI":"10.1038\/srep27663","article-title":"The use of NS1 rapid diagnostic test and qRT-PCR to complement IgM ELISA for improved dengue diagnosis from single specimen","volume":"6","author":"Teoh","year":"2016","journal-title":"Sci Rep"},{"key":"10.4103\/ijmm.IJMM_20_249_bib4","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s13337-018-0423-y","article-title":"Simultaneous detection and serotyping of dengue infection using single tube multiplex CDC Dengue real-time RT-PCR from India","volume":"29","author":"Sharma","year":"2018","journal-title":"Virusdisease"},{"key":"10.4103\/ijmm.IJMM_20_249_bib5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4103\/ijmm.IJMM_17_427","article-title":"Identification of prevalent dengue serotypes by reverse transcriptase polymerase chain reaction and correlation with severity of dengue as per the recent World Health Organization classification (2009)","volume":"36","author":"Mehta","year":"2018","journal-title":"Indian J Med Microbiol"},{"key":"10.4103\/ijmm.IJMM_20_249_bib6","doi-asserted-by":"crossref","first-page":"e0006618","DOI":"10.1371\/journal.pntd.0006618","article-title":"Dengue infection in India: A systematic review and meta-analysis","volume":"12","author":"Ganeshkumar","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"10.4103\/ijmm.IJMM_20_249_bib7","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4103\/0255-0857.115640","article-title":"Assessing effect of climate on the incidence of dengue in Tamil Nadu","volume":"31","author":"Chandy","year":"2013","journal-title":"Indian J Med Microbiol"},{"key":"10.4103\/ijmm.IJMM_20_249_bib8","first-page":"755","article-title":"Evaluation of recent WHO classification (2009) for assessment of dengue disease severity","volume":"4","author":"Mehta","year":"2015","journal-title":"Int J Curr Microbiol Appl Sci"},{"key":"10.4103\/ijmm.IJMM_20_249_bib9","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s12985-017-0768-7","article-title":"The prevalence of dengue virus serotypes in asymptomatic blood donors reveals the emergence of serotype 4 in Saudi Arabia","volume":"14","author":"Ashshi","year":"2017","journal-title":"Virol J"},{"key":"10.4103\/ijmm.IJMM_20_249_bib10","doi-asserted-by":"crossref","first-page":"e0004497","DOI":"10.1371\/journal.pntd.0004497","article-title":"Immune response to dengue virus infection in pediatric patients in New Delhi, India-Association of viremia, inflammatory mediators and monocytes with disease severity","volume":"10","author":"Singla","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"10.4103\/ijmm.IJMM_20_249_bib11","doi-asserted-by":"crossref","first-page":"e2311","DOI":"10.1371\/journal.pntd.0002311","article-title":"Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus","volume":"7","author":"Santiago","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"10.4103\/ijmm.IJMM_20_249_bib12","first-page":"1","article-title":"Clinical profile of dengue fever in children: A study from Southern Odisha, India","volume":"2016","author":"Mishra","year":"2016","journal-title":"Scientifica"},{"key":"10.4103\/ijmm.IJMM_20_249_bib13","doi-asserted-by":"crossref","first-page":"115","DOI":"10.4103\/0974-777X.188596","article-title":"Clinically profiling pediatric patients with dengue","volume":"8","author":"Pothapregada","year":"2016","journal-title":"J Glob Infect Dis"},{"key":"10.4103\/ijmm.IJMM_20_249_bib14","doi-asserted-by":"crossref","first-page":"3919","DOI":"10.1128\/JCM.41.8.3919-3921.2003","article-title":"Dengue disease spectrum among infants in the 2001 dengue epidemic in Chennai, Tamil Nadu, India","volume":"41","author":"Kabilan","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.4103\/ijmm.IJMM_20_249_bib15","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1002\/jmv.21290","article-title":"A wide spectrum of dengue IgM and PCR positivity post-onset of illness found in a large dengue 3 outbreak in Pakistan","volume":"80","author":"Tang","year":"2008","journal-title":"J Med Virol"},{"key":"10.4103\/ijmm.IJMM_20_249_bib16","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.20546\/ijcmas.2019.801.139","article-title":"Co-Circulation of dengue serotypes in a tertiary care hospital in NCR region","volume":"8","author":"Tomar","year":"2019","journal-title":"Int J Curr Microbiol App Sci"},{"key":"10.4103\/ijmm.IJMM_20_249_bib17","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4103\/ijmm.IJMM_18_109","article-title":"Co-circulation of four dengue serotypes at South Eastern Andhra Pradesh, India: A prospective study","volume":"36","author":"Racherla","year":"2018","journal-title":"Indian J Med Microbiol"},{"key":"10.4103\/ijmm.IJMM_20_249_bib18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4172\/2329-8731.1000177","article-title":"Molecular epidemiology of dengue virus genotype circulation in Tamil Nadu, India, 2011-2014","volume":"6","author":"Ramalingam","year":"2018","journal-title":"J Infect Dis Preve Med"}],"container-title":["Indian Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085720315346?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085720315346?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T19:18:04Z","timestamp":1759346284000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0255085720315346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":18,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["S0255085720315346"],"URL":"https:\/\/doi.org\/10.4103\/ijmm.ijmm_20_249","relation":{},"ISSN":["0255-0857"],"issn-type":[{"value":"0255-0857","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Utility of a Multiplex Real-Time Polymerase Chain Reaction for Combined Detection and Serotyping of Dengue Virus in Paediatric Patients Hospitalised with Severe Dengue: A Report from Chennai","name":"articletitle","label":"Article Title"},{"value":"Indian Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.4103\/ijmm.IJMM_20_249","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2020 Indian Journal of Medical Microbiology. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}