{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:19:53Z","timestamp":1777421993849,"version":"3.51.4"},"reference-count":26,"publisher":"Scientific Scholar","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,1]]},"abstract":"\n Background & objectives:<\/jats:title>\n Human cryptosporidiosis is endemic worldwide, and at least eight species have been reported in humans; the most common being Cryptosporidium hominis<\/jats:italic> and C. parvum<\/jats:italic>. Detailed understanding of the epidemiology of Cryptosporidium<\/jats:italic> is increasingly facilitated using standardized universal technique for species differentiation and subtyping. In this study micro- and minisatellite targets in chromosome 6 were used to assess genetic diversity of C. hominis<\/jats:italic> by sequence length polymorphisms along with single nucleotide polymorphisms (SNPs).<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n A total of 84 Cryptosporidium<\/jats:italic> positive stool specimens were subjected to speciation and genotyping using small subunit (SSU) ribosomal RNA (rRNA) as the target gene. Genetic heterogeneity amongst C. hominis<\/jats:italic> isolates was assessed by sequencing minisatellites, microsatellites and polymorphic markers including genes encoding the 60 kDa glycoprotein (GP60), a 47 kDa protein (CP47), a mucin-like protein (Mucin-1), a serine repeat antigen (MSC6-7) and a 56 kDa transmembrane protein (CP56).<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Of the 84 Cryptosporidium<\/jats:italic> positive stool specimens, 77 (92%) were positive by SSU rRNA gene polymerase chain reaction (PCR) assay. Of these 77 isolates, 54 were identified as C. hominis<\/jats:italic> and 23 as C. parvum<\/jats:italic>. Of all the loci studied by multilocus sequence typing (MLST), GP60 gene could reveal the highest genetic diversity. Population substructure analysis of C. hominis<\/jats:italic> performed by combined sequence length and nucleotide polymorphism showed nine multilocus subtypes, all of which were distinct groups in the study population.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n MLST, a powerful discriminatory test, demonstrated both variations and distribution pattern of Cryptosporidium<\/jats:italic> species and its subtypes.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_1064_14","type":"journal-article","created":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T12:31:30Z","timestamp":1496061090000},"page":"102-111","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":10,"title":["Multilocus sequence typing of Cryptosporidium hominis from northern 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