{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:20:43Z","timestamp":1792740043651,"version":"4.5.0"},"reference-count":22,"publisher":"Scientific Scholar","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,10]]},"abstract":"\n Background & objectives:<\/jats:title>\n Human dirofilariosis is a well-recognized zoonosis caused by several species of the genus Dirofilaria<\/jats:italic>. The disease is prevalent among canines and human beings in Kerala. The objective of the present study was to confirm the human Dirofilaria<\/jats:italic> isolates by molecular characterization.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n The worms or segments obtained from human sources were subjected to diagnostic polymerase chain reaction (PCR) targeting the cytochrome oxidase subunit 1 (COI<\/jats:italic>) and 5S ribosomal RNA (rRNA<\/jats:italic>) genes of Dirofilaria repens<\/jats:italic>. The amplicons were sequenced and analyzed.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n The filariid nematodes recovered from ocular as well as subcutaneous tissue of human origin were identified as D. repens<\/jats:italic> based on PCR targeting COI<\/jats:italic> as well as 5S rRNA<\/jats:italic> genes. The phylogenetic analysis of the COI<\/jats:italic> gene nucleotide sequence obtained in the present study showed that D. repens<\/jats:italic> shared the closest evolutionary relationship with D. honkongensis<\/jats:italic>.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n Molecular identification of D. repens<\/jats:italic> isolated from human source assumes significance from the point of zoonotic threat of this mosquito-borne nematode. Phylogenetic analysis revealed a close evolutionary relationship with Asian isolate of D. honkongensis<\/jats:italic>. Timely detection and treatment of infection in dogs, together with mosquito control, should be an integral part of the control strategy of this disease.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_1425_15","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T19:24:47Z","timestamp":1734636287000},"page":"528-533","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Molecular characterization of human Dirofilaria isolates from Kerala"],"prefix":"10.25259","volume":"146","author":[{"given":"Najuma","family":"Nazar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bindu","family":"Lakshmanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.K.","family":"Jayavardhanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","reference":[{"key":"R1-13-20241212","doi-asserted-by":"crossref","first-page":"3534","DOI":"10.1128\/JCM.01590-12","article-title":"A novel Dirofilaria species causing human and canine infections in Hong Kong","volume":"50","author":"To","year":"2012","journal-title":"J Clin Microbiol"},{"key":"R2-13-20241212","first-page":"2","article-title":"Human dirofilariasis: An emerging zoonosis","volume":"3","author":"Reddy","year":"2013","journal-title":"Trop 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