{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T07:54:16Z","timestamp":1777535656160,"version":"3.51.4"},"reference-count":24,"publisher":"Scientific Scholar","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives<\/jats:title>\n            <jats:p>Polio, measles, rubella, influenza and rotavirus surveillance programmes are of great public health importance globally. Virus isolation using cell culture is an integral part of such programmes. Possibility of unintended isolation of SARS-CoV-2 from clinical specimens processed in biosafety level-2 (BSL-2) laboratories during the above-mentioned surveillance programmes, cannot be ruled out. The present study was conducted to assess the susceptibility of different cell lines to SARS-CoV-2 used in these programmes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Replication of SARS-CoV-2 was studied in RD and L20B, Vero\/hSLAM, MA-104 and Madin\u2013Darby Canine Kidney (MDCK) cell lines, used for the isolation of polio, measles, rubella, rotavirus and influenza viruses, respectively. SARS-CoV-2 at 0.01 multiplicity of infection was inoculated and the viral growth was assessed by observation of cytopathic effects followed by real-time reverse transcription\u2013polymerase chain reaction (qRT-PCR). Vero CCL-81 cell line was used as a positive control.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>SARS-CoV-2 replicated in Vero\/hSLAM, and MA-104 cells, whereas it did not replicate in L20B, RD and MDCK cells. Vero\/hSLAM, and Vero CCL-81 showed rounding, degeneration and detachment of cells; MA-104 cells also showed syncytia formation. In qRT-PCR, Vero\/hSLAM and MA-104 showed 10<jats:sup>6<\/jats:sup> and Vero CCL-81 showed 10<jats:sup>7<\/jats:sup> viral RNA copies per \u00b5l. The 50 per cent tissue culture infectious dose titres of Vero\/hSLAM, MA-104 and Vero CCL-81 were 10<jats:sup>5.54<\/jats:sup>, 10<jats:sup>5.29<\/jats:sup> and 10<jats:sup>6.45<\/jats:sup>\/ml, respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions<\/jats:title>\n            <jats:p>Replication of SARS-CoV-2 in Vero\/hSLAM and MA-104 underscores the possibility of its unintended isolation during surveillance procedures aiming to isolate measles, rubella and rotavirus. This could result in accidental exposure to high titres of SARS-CoV-2, which can result in laboratory acquired infections and community risk, highlighting the need for revisiting biosafety measures in public health laboratories.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_1448_21","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T09:27:13Z","timestamp":1659432433000},"page":"129-135","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Replication of SARS-CoV-2 in cell lines used in public health surveillance programmes with special emphasis on biosafety"],"prefix":"10.25259","volume":"155","author":[{"given":"Shailesh D.","family":"Pawar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadhana S.","family":"Kode","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachin S.","family":"Keng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deeksha S.","family":"Tare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ousmane M.","family":"Diop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priya","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepa K.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucky","family":"Sangal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pragya D.","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varsha A.","family":"Potdar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R5-19-20241212","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1111\/codi.15138","article-title":"Persistent viral shedding of SARS-CoV-2 in faeces \u2013A rapid review","volume":"22","author":"Gupta","year":"2020","journal-title":"Colorectal Dis"},{"key":"R8-19-20241212","doi-asserted-by":"crossref","first-page":"244","DOI":"10.4103\/ijmr.IJMR_1029_20","article-title":"First isolation of SARS-CoV-2 from clinical samples in India","volume":"151","author":"Sarkale","year":"2020","journal-title":"Indian J Med Res"},{"key":"R9-19-20241212","doi-asserted-by":"crossref","first-page":"70","DOI":"10.4103\/ijmr.IJMR_2257_20","article-title":"Transcriptome &viral growth analysis of SARS-CoV-2-infected Vero CCL-81 cells","volume":"152","author":"Nyayanit","year":"2020","journal-title":"Indian J Med Res"},{"key":"R10-19-20241212","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am J Epidemiol"},{"key":"R11-19-20241212","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4103\/ijmr.IJMR_671_20","article-title":"Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India","volume":"151","author":"Choudhary","year":"2020","journal-title":"Indian J Med Res"},{"key":"R12-19-20241212","doi-asserted-by":"crossref","first-page":"459","DOI":"10.5694\/mja2.50569","article-title":"Isolation and rapid sharing of the 2019 novel coronavirus (SARS-CoV-2) from the first patient diagnosed with COVID-19 in Australia","volume":"212","author":"Caly","year":"2020","journal-title":"Med J Aust"},{"key":"R13-19-20241212","doi-asserted-by":"crossref","first-page":"e0238614","DOI":"10.1371\/journal.pone.0238614","article-title":"Isolation and characterization of severe acute respiratory syndrome coronavirus 2 in Turkey","volume":"15","author":"Pavel","year":"2020","journal-title":"PLoS One"},{"key":"R14-19-20241212","doi-asserted-by":"crossref","first-page":"1380","DOI":"10.3201\/eid2705.210023","article-title":"Susceptibility to SARS-CoV-2 of cell lines and substrates commonly used to diagnose and isolate influenza and other viruses","volume":"27","author":"Wang","year":"2021","journal-title":"Emerg Infect Dis"},{"key":"R15-19-20241212","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.3201\/eid2606.200516","article-title":"Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States","volume":"26","author":"Harcourt","year":"2020","journal-title":"Emerg Infect Dis"},{"key":"R16-19-20241212","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1016\/j.cell.2021.02.042","article-title":"SARS-CoV-2 501Y. 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