{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:56:22Z","timestamp":1791600982541,"version":"4.3.4"},"reference-count":20,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives:<\/jats:title>\n            <jats:p>Acetylcholinesterase (AChE) inhibitors represent a major class of drugs which provide symptomatic relief and improvement in cognitive function in Alzheimer's disease (AD). In this study, cubebin, a dibenzylbutyrolactone lignan, was isolated from <jats:italic toggle=\"yes\">Piper cubeba<\/jats:italic> and investigated for its AChE inhibitory activity in an attempt to explore its potential for memory-enhancing activities in mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Molecular docking of cubebin was carried out followed by <jats:italic toggle=\"yes\">in vitro<\/jats:italic> AChE activity. Mice were treated with cubebin (25 &amp; 50 mg\/kg; i.p.), for three days and memory impairment was induced by scopolamine (3 mg\/kg; i.p.). Memory function was evaluated by Morris water maze (MWM) test. Biochemical parameters of oxidative stress and cholinergic function were estimated in brain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>Molecular docking study revealed that cubebin was well bound within the binding site of the AChE enzyme showing interactions such as \u03c0-\u03c0 stacking and hydrogen bonding with residues present therein. Cubebin inhibited AChE enzyme in an <jats:italic toggle=\"yes\">in vitro<\/jats:italic> assay with IC<jats:sub>50<\/jats:sub> value of 992 \u03bcM. Scopolamine administration caused a significant impairment of learning and memory in mice, as indicated by a marked decrease in MWM performance. Scopolamine administration also produced a significant enhancement of brain AChE activity and oxidative stress in mice brain. Pre-treatment of cubebin (25 and 50 mg\/kg; i.p.) significantly prevented scopolamine-induced learning and memory deficits along with attenuation of scopolamine-induced rise in brain AChE activity and oxidative stress level.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions:<\/jats:title>\n            <jats:p>Cubebin showed promising protective activity in scopolamine-induced spatial memory impairment in mice. This could be attributed to its brain AChE inhibition and antioxidant activity.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_156_14","type":"journal-article","created":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T10:21:27Z","timestamp":1513765287000},"page":"255-259","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":26,"title":["Neuroprotective effect of Cubebin"],"prefix":"10.25259","volume":"146","author":[{"given":"Gauresh S.","family":"Somani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mruniya S.","family":"Nahire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aakash D.","family":"Parikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandar B.","family":"Mulik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya J.","family":"Ghumatkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirti S.","family":"Laddha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadhana","family":"Sathaye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","reference":[{"key":"R1-15-20241212","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4103\/0019-5545.44908","article-title":"Neurobiology of Alzheimer's disease","volume":"51","author":"Mohandas","year":"2009","journal-title":"Indian J Psychiatry"},{"key":"R2-15-20241212","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2147\/BTAT.S17297","article-title":"Plant-derived acetylcholinesterase inhibitory alkaloids for the treatment of Alzheimer's 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mice"],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.4103\/ijmr.ijmr_156_14","relation":{},"ISSN":["0971-5916"],"issn-type":[{"value":"0971-5916","type":"print"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}