{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:52:43Z","timestamp":1767181963616,"version":"3.41.0"},"reference-count":30,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives:<\/jats:title>\n            <jats:p>\n                                 <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic> (pneumococcus) is a highly invasive extracellular pathogen that causes diseases such as pneumonia, otitis media and meningitis. This study was undertaken to determine the serotype diversity and penicillin susceptibility of <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic> isolated from paediatric patients in a tertiary teaching hospital in Malaysia.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>A total of 125 clinical isolates collected from January 2013 to May 2015 were serotyped using seven sequential multiplex polymerase chain reactions. The susceptibility of these isolates to penicillin was also investigated.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>Serotypes detected among the isolates were serotypes 3, 6A\/B, 6C, 11\/A\/D\/F, 15A\/F, 19A, 19F, 23A, 23F, 34. Serotypes 19F and 6A\/B were the most prevalent serotypes detected. Most of the <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic> were isolated from nasopharyngeal samples of children below five years of age. Majority of the isolates were penicillin susceptible. Only 5.6 per cent of the isolates were non-susceptible to penicillin, mostly of serotype 19F.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions:<\/jats:title>\n            <jats:p>Our study revealed the distribution of various serotypes in <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic> isolates obtained from children in a teaching hospital at Kuala Lumpur, Malaysia and decreasing rates of penicillin resistance among them. The shifts in serotypes and susceptibility to penicillin from time to time have been observed. Continuous monitoring and surveillance are pivotal for better infection control and management of pneumococcal infections among children.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_1987_16","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T04:55:27Z","timestamp":1539924927000},"page":"225-231","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Serotypes &amp; penicillin susceptibility of Streptococcus pneumoniae isolated from children admitted to a tertiary teaching hospital in Malaysia"],"prefix":"10.25259","volume":"148","author":[{"given":"Prasanna","family":"Subramaniam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kartini Abdul","family":"Jabar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boon Pin","family":"Kee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Wie","family":"Chong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Marie","family":"Nathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessie","family":"de Bruyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surendran","family":"Thavagnanam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kek Heng","family":"Chua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd Yasim","family":"Md Yusof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy Shuan Ju","family":"Teh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R1-14-20241212","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1128\/mr.59.4.591-603.1995","article-title":"Streptococcus pneumoniae: Virulence factors, pathogenesis, and vaccines","volume":"59","author":"AlonsoDeVelasco","year":"1995","journal-title":"Microbiol Rev"},{"key":"R2-14-20241212","first-page":"S134","article-title":"Antibiotic resistance and the potential impact of pneumococcal conjugate vaccines","volume":"27","author":"Dagan","year":"2003","journal-title":"Commun Dis Intell Q Rep"},{"key":"R3-14-20241212","doi-asserted-by":"crossref","first-page":"4881","DOI":"10.1016\/j.vaccine.2013.07.054","article-title":"Serotype prevalence and antibiotic resistance in Streptococcus pneumoniae clinical isolates among global populations","volume":"31","author":"Hackel","year":"2013","journal-title":"Vaccine"},{"key":"R4-14-20241212","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1086\/322048","article-title":"Nasopharyngeal carriage of Streptococcus pneumoniae in Finnish children younger than 2 years old","volume":"184","author":"Syrj\u00e4nen","year":"2001","journal-title":"J Infect Dis"},{"key":"R5-14-20241212","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1038\/emi.2014.37","article-title":"Genetic diversity of Streptococcus pneumoniae causing meningitis and sepsis in Singapore during the first year of PCV7 implementation","volume":"3","author":"Jauneikaite","year":"2014","journal-title":"Emerg Microbes Infect"},{"key":"R6-14-20241212","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1128\/JCM.01937-09","article-title":"Association of serotypes of Streptococcus pneumoniae with age in invasive pneumococcal disease","volume":"48","author":"Im\u00f6hl","year":"2010","journal-title":"J Clin Microbiol"},{"key":"R7-14-20241212","first-page":"e51208","article-title":"Capsular serotyping of Streptococcus pneumoniae using the quellung reaction","volume":"(84)","author":"Habib","year":"2014","journal-title":"J Vis Exp"},{"key":"R8-14-20241212","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1128\/jcm.18.4.978-980.1983","article-title":"Serotyping of Streptococcus pneumoniae strains by coagglutination and counterimmunoelectrophoresis","volume":"18","author":"Trollfors","year":"1983","journal-title":"J Clin Microbiol"},{"key":"R9-14-20241212","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1128\/JCM.42.6.2518-2522.2004","article-title":"Simple, rapid latex agglutination test for serotyping of pneumococci (Pneumotest-latex)","volume":"42","author":"Slotved","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R10-14-20241212","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/JCM.44.1.124-131.2006","article-title":"Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates","volume":"44","author":"Pai","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R12-14-20241212","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1080\/20477724.2016.1229884","article-title":"Intestinal carriage of multidrug-resistant gram-negative bacteria in preterm-infants during hospitalization in neonatal Intensive Care Unit (NICU)","volume":"110","author":"Yap","year":"2016","journal-title":"Pathog Glob Health"},{"key":"R13-14-20241212","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1099\/00221287-144-11-3049","article-title":"A multilocus sequence typing scheme for Streptococcus pneumoniae: Identification of clones associated with serious invasive disease","volume":"144","author":"Enright","year":"1998","journal-title":"Microbiology"},{"key":"R14-14-20241212","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1128\/JCM.01919-08","article-title":"PCR-based quantitation and clonal diversity of the current prevalent invasive serogroup 6 pneumococcal serotype, 6C, in the United States in 1999 and 2006 to 2007","volume":"47","author":"Carvalho Mda","year":"2009","journal-title":"J Clin Microbiol"},{"key":"R15-14-20241212","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1128\/JCM.02243-09","article-title":"Revisiting pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes","volume":"48","author":"da Gloria Carvalho","year":"2010","journal-title":"J Clin Microbiol"},{"key":"R16-14-20241212","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1099\/jmm.0.47347-0","article-title":"Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children","volume":"56","author":"Dias","year":"2007","journal-title":"J Med Microbiol"},{"key":"R17-14-20241212","doi-asserted-by":"crossref","first-page":"e3576","DOI":"10.1371\/journal.pone.0003576","article-title":"Identification of serotype in culture negative pneumococcal meningitis using sequential multiplex PCR: Implication for surveillance and vaccine design","volume":"3","author":"Saha","year":"2008","journal-title":"PLoS One"},{"key":"R18-14-20241212","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/1471-2334-11-100","article-title":"Sequential multiplex PCR assay for determining capsular serotypes of colonizing S.pneumoniae","volume":"11","author":"Jourdain","year":"2011","journal-title":"BMC Infect Dis"},{"key":"R19-14-20241212","doi-asserted-by":"crossref","first-page":"4","DOI":"10.3346\/jkms.2013.28.1.4","article-title":"Clinical implications of pneumococcal serotypes: Invasive disease potential, clinical presentations, and antibiotic resistance","volume":"28","author":"Song","year":"2013","journal-title":"J Korean Med Sci"},{"key":"R20-14-20241212","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1099\/jmm.0.2008\/000935-0","article-title":"Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children","volume":"57","author":"Azzari","year":"2008","journal-title":"J Med Microbiol"},{"key":"R21-14-20241212","doi-asserted-by":"crossref","first-page":"3209","DOI":"10.1128\/JCM.00610-11","article-title":"Serotyping of Streptococcus pneumoniae isolates from nasopharyngeal samples: Use of an algorithm combining microbiologic, serologic, and sequential multiplex PCR techniques","volume":"49","author":"Miernyk","year":"2011","journal-title":"J Clin Microbiol"},{"key":"R22-14-20241212","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1086\/650534","article-title":"Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates that cause invasive disease among Chinese children","volume":"50","author":"Xue","year":"2010","journal-title":"Clin Infect Dis"},{"key":"R23-14-20241212","doi-asserted-by":"crossref","first-page":"34","DOI":"10.2807\/1560-7917.ES2015.20.13.21079","article-title":"Streptococcus pneumoniae nasopharyngeal colonisation in children aged under six years with acute respiratory tract infection in Lithuania, February 2012 to March 2013","volume":"20","author":"Usonis","year":"2015","journal-title":"Euro Surveill"},{"key":"R24-14-20241212","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ijid.2013.11.005","article-title":"Serotypes and antibiotic resistance of non-invasive Streptococcus pneumoniae circulating in pediatric hospitals in Moscow, Russia","volume":"20","author":"Mayanskiy","year":"2014","journal-title":"Int J Infect Dis"},{"key":"R25-14-20241212","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3126\/kumj.v8i2.3551","article-title":"Antimicrobial susceptibility pattern and serotyping of Streptococcus pneumoniae isolated from Kanti children hospital in Nepal","volume":"8","author":"Rijal","year":"2010","journal-title":"Kathmandu Univ Med J (KUMJ"},{"key":"R26-14-20241212","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0140-6736(09)61204-6","article-title":"Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: Global estimates","volume":"374","author":"O\u2019Brien","year":"2009","journal-title":"Lancet"},{"key":"R27-14-20241212","doi-asserted-by":"crossref","first-page":"5688","DOI":"10.1016\/j.vaccine.2011.06.004","article-title":"Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals","volume":"29","author":"Yasin","year":"2011","journal-title":"Vaccine"},{"key":"R28-14-20241212","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1016\/j.vaccine.2012.03.066","article-title":"Prevalence of Streptococcus pneumoniae serotypes causing invasive and non-invasive disease in South East Asia: A review","volume":"30","author":"Jauneikaite","year":"2012","journal-title":"Vaccine"},{"key":"R29-14-20241212","doi-asserted-by":"crossref","first-page":"e19547","DOI":"10.1371\/journal.pone.0019547","article-title":"Capsular serotype and antibiotic resistance of Streptococcus pneumoniae isolates in Malaysia","volume":"6","author":"Le","year":"2011","journal-title":"PLoS One"},{"key":"R30-14-20241212","first-page":"338","article-title":"Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae","volume":"30","author":"Shakrin","year":"2013","journal-title":"Trop Biomed"},{"key":"R31-14-20241212","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/S1201-9712(03)90051-8","article-title":"Penicillin susceptibility and molecular characteristics of clinical isolates of Streptococcus pneumoniae at the university of Malaya medical center, Kuala Lumpur, Malaysia","volume":"7","author":"Desa","year":"2003","journal-title":"Int J Infect Dis"}],"container-title":["Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/02223309-201848020-00014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T23:00:38Z","timestamp":1751497238000},"score":1,"resource":{"primary":{"URL":"https:\/\/ijmr.org.in\/serotypes-penicillin-susceptibility-of-streptococcus-pneumoniae-isolated-from-children-admitted-to-a-tertiary-teaching-hospital-in-malaysia\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018]]}},"URL":"https:\/\/doi.org\/10.4103\/ijmr.ijmr_1987_16","relation":{},"ISSN":["0971-5916"],"issn-type":[{"type":"print","value":"0971-5916"}],"subject":[],"published":{"date-parts":[[2018,8]]}}}