{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:55:08Z","timestamp":1757314508327,"version":"3.41.0"},"reference-count":24,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2]]},"abstract":"\n Background & objectives:<\/jats:title>\n \n Pediococcus pentosaceus<\/jats:italic> has been reported to cause clinical infections while it is being promoted as probiotic in food formulations. Antibiotic resistance (AR) genes in this species are a matter of concern for treating clinical infections. The present study was aimed at understanding the phenotypic resistance of P. pentosaceus<\/jats:italic> to macrolide-lincosamide-streptogramin B (MLSB<\/jats:sub>) antibiotics and the transfer of AR to pathogens.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n P. pentosacues isolates (n=15) recovered from fermented foods were screened for phenotypic resistance to MLSB<\/jats:sub> antibiotics using disc diffusion and microbroth dilution methods. Localization and transferability of the identified resistance genes, erm<\/jats:italic>(B) and msr<\/jats:italic>(C) were evaluated through Southern hybridization and in vitro<\/jats:italic> conjugation methods.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Four different phenotypes; sensitive (S) (n=5), macrolide (M) (n=7), lincosamide (L) (n=2) and constitutive (cMLSB<\/jats:sub>) (n=1) were observed among the 15 P. pentosaceus<\/jats:italic> isolates. High-level resistance (>256 \u03bcg\/ml) to MLSB<\/jats:sub> was observed with one cMLSB<\/jats:sub> phenotypic isolate IB6-2A. Intermediate resistance (8-16 \u03bcg\/ml) to macrolides and lincosamides was observed among M and L phenotype isolates, respectively. Cultures with S phenotype were susceptible to all other antibiotics but showed unusual minimum inhibitory concentration (MIC) values of 8-16 \u03bcg\/ml for azithromycin. Southern hybridization studies revealed that resistance genes localized on the plasmids could be conjugally transferred to Enterococcus faecalis<\/jats:italic> JH2-2.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The study provides insights into the emerging novel resistance patterns in P. pentosaceus<\/jats:italic> and their ability to disseminate AR. Monitoring their resistance phenotypes before use of MLS antibiotics can help in successful treatment of Pediococcal infections in humans.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_2055_17","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T07:30:52Z","timestamp":1559547052000},"page":"270-275","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Heterogeneity of macrolide-lincosamide-streptogramin phenotype & conjugal transfer of erm(B) in Pediococcus pentosaceus"],"prefix":"10.25259","volume":"149","author":[{"given":"Surya Chandra Rao","family":"Thumu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash 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