{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:53:16Z","timestamp":1792291996276,"version":"4.4.0"},"reference-count":31,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives:<\/jats:title>\n            <jats:p>Infection from fluoroquinolone-resistant extra-intestinal <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic> is a global concern. In this study, isolation and characterization of fluoroquinolone-resistant extra-intestinal <jats:italic toggle=\"yes\">E. coli<\/jats:italic> isolates obtained from hospital samples were undertaken to detect plasmid-mediated quinolone resistance (<jats:italic toggle=\"yes\">PMQR<\/jats:italic>) genes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Forty three isolates of <jats:italic toggle=\"yes\">E. coli<\/jats:italic> obtained from patients with extra-intestinal infections were subjected to antibiogram to detect fluoroquinolone resistance. The mechanism of fluoroquinolone resistance was determined by the detection of PMQR genes and mutations in quinolone resistance determining region (QRDR).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>Of the 43 isolates, 36 were resistant to nalidixic acid (83.72%) and 28 to ciprofloxacin (65.11%). Eight <jats:italic toggle=\"yes\">E. coli<\/jats:italic> isolates showed total resistance to both the antimicrobials without any minimum inhibitory concentration. The detection of PMQR genes with <jats:italic toggle=\"yes\">qnr<\/jats:italic> primers showed the presence of <jats:italic toggle=\"yes\">qnrA<\/jats:italic> in two, <jats:italic toggle=\"yes\">qnrB<\/jats:italic> in six and <jats:italic toggle=\"yes\">qnrS<\/jats:italic> in 21 isolates. The gene coding for quinolone efflux pump (<jats:italic toggle=\"yes\">qepA<\/jats:italic>) was not detected in any of the isolates tested. The presence of some unexpressed PMQR genes in fluoroquinolone sensitive isolates was also observed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions:<\/jats:title>\n            <jats:p>The detection of silent PMQR genes as observed in the present study presents a risk of the transfer of the silent resistance genes to other microorganisms if present in conjugative plasmids, thus posing a therapeutic challenge to the physicians. Hence, frequent monitoring is to be done for all resistance determinants.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_2092_17","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T07:30:52Z","timestamp":1559547052000},"page":"192-198","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":20,"title":["Plasmid-mediated fluoroquinolone resistance associated with extra-intestinal Escherichia coli isolates from hospital samples"],"prefix":"10.25259","volume":"149","author":[{"given":"Shruthi S.","family":"Shetty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijaya 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