{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:29:29Z","timestamp":1787030969886,"version":"build-2736575974"},"reference-count":31,"publisher":"Scientific Scholar","issue":"Suppl 1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,7]]},"abstract":"\n Background & objectives:<\/jats:title>\n \n The emergence and rapid spread of carbapenem resistance mediated by metallo-beta-lactamase (MBL) in\n Pseudomonas aeruginosa<\/jats:italic>\n is of major concern due to limited therapeutic options. This study was aimed at detecting the presence of MBL and its association with integrons in imipenem-resistant\n P. aeruginosa<\/jats:italic>\n isolates and to determine their genetic relatedness.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n A total of 213\n P. aeruginosa<\/jats:italic>\n isolates were collected from two tertiary care centres and tested against anti-pseudomonal antibiotics by antimicrobial susceptibility testing, followed by the detection of MBL production by combined disk method. Minimum inhibitory concentration (MIC) of meropenem was determined by E-test. Multiplex polymerase chain reaction (PCR) was performed for the detection of\n bla<\/jats:italic>\n SPM<\/jats:sub>\n , bla<\/jats:italic>\n IMP<\/jats:sub>\n ,\n bla<\/jats:italic>\n VIM<\/jats:sub>\n ,\n bla<\/jats:italic>\n NDM<\/jats:sub>\n ,\n bla<\/jats:italic>\n GIM<\/jats:sub>\n and\n bla<\/jats:italic>\n SIM<\/jats:sub>\n . PCR was carried out to characterize the variable region of class 1 integron. Transcongujation assay was carried out for the confirmation of plasmid-mediated resistance. Enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR was performed for determining the genetic relatedness among\n P. aeruginosa<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Of the 213\n P. aeruginosa<\/jats:italic>\n isolates, 22 (10%) were found to be carbapenem resistant and these were from pus 18 (82%), urine 2 (9%), sputum 1 (5%) and tracheal wash 1 (5%). Among 22 isolates, 18 (81.8%) were found to be MBL producers by phenotypic method and MIC range of meropenem was 8 to >32 \u00b5g\/ml. PCR amplification showed that 20 (91%) isolates carried any one of the MBL genes tested:\n bla<\/jats:italic>\n VIM<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM<\/jats:sub>\n in seven (32%) and six (27%) isolates, respectively;\n bla<\/jats:italic>\n VIM<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM<\/jats:sub>\n in three (14%);\n bla<\/jats:italic>\n IMP<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM<\/jats:sub>\n in two (9%);\n bla<\/jats:italic>\n VIM<\/jats:sub>\n and\n bla<\/jats:italic>\n IMP<\/jats:sub>\n in one (5%) isolate. The\n bla<\/jats:italic>\n VIM<\/jats:sub>\n ,\n bla<\/jats:italic>\n IMP<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM<\/jats:sub>\n were found to co-exist in one isolate. None of the isolates were positive for\n bla<\/jats:italic>\n SPM<\/jats:sub>\n ,\n bla<\/jats:italic>\n SIM<\/jats:sub>\n and\n bla<\/jats:italic>\n GIM<\/jats:sub>\n . All 22 isolates carried class I integron. Of the 20 MBL-positive isolates, transconjugants were obtained for 15 isolates. ERIC-PCR analysis showed all isolates to be clonally independent.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n \n Our results showed 10.3 per cent of carbapenem resistance among\n P. aeruginosa<\/jats:italic>\n isolates, and the coexistence of MBL-encoding genes among\n P. aeruginosa<\/jats:italic>\n mediated by class I integron.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_29_16","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:53:27Z","timestamp":1513904007000},"page":"S46-S52","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":24,"title":["Coexistence of metallo-beta-lactamase-encoding genes in Pseudomonas aeruginosa"],"prefix":"10.25259","volume":"146","author":[{"given":"Lavanya","family":"Mohanam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangam","family":"Menon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","reference":[{"key":"R1-7-20241212","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1093\/jac\/dkp201","article-title":"Update on the treatment of Pseudomonas aeruginosa pneumonia","volume":"64","author":"El Solh","year":"2009","journal-title":"J Antimicrob Chemother"},{"key":"R2-7-20241212","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.diagmicrobio.2012.12.012","article-title":"Activities of carbapenem and comparator agents against contemporary US Pseudomonas aeruginosa isolates from the CAPITAL surveillance program","volume":"75","author":"Morrow","year":"2013","journal-title":"Diagn Microbiol Infect 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