{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T06:03:39Z","timestamp":1778997819352,"version":"3.51.4"},"reference-count":15,"publisher":"Scientific Scholar","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,3]]},"abstract":"\n Background & objectives:<\/jats:title>\n Shiga toxin (Stx) is produced by Shigella dysenteriae<\/jats:italic>, a Gram-negative, facultative anaerobic bacillus that causes shigellosis, haemolytic uraemic syndrome (HUS) and Reiter's syndrome. The detection methods for shiga toxin needs to be rapid, accurate, reliable and must be extensively evaluated under field conditions. The aim of this study was to develop rapid, sensitive and specific detection method for Stx.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Mice and rabbits were immunized with purified recombinant Shiga toxin B (rStxB). Using these antibodies dot ELISA, sandwich ELISA and flow through assay were developed.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n The high-titre antibodies specifically reacted with purified rStxB. Dot-ELISA, sandwich ELISA and flow-through assay were developed and standardized that could detect StxB with limit of detection (LOD) of 9.75, 9.7 ng\/ml and 0.46 \u03bcg\/cassette, respectively.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The rStxB was used to produce antibodies to avoid handling of pathogen. The Flow through assay \u2018developed was specific, rapid and field amenable.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_308_17","type":"journal-article","created":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T08:11:27Z","timestamp":1561363887000},"page":"412-417","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Immunological detection assays for recombinant Shiga toxin & Shigella dysenteriae"],"prefix":"10.25259","volume":"149","author":[{"given":"Pallavi","family":"Gupta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram Kumar","family":"Dhaked","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R1-18-20241212","first-page":"157","article-title":"Shiga toxin and tetanus toxin as a potential biologic weapon","volume":"39","author":"Toczyska","year":"2015","journal-title":"Pol Merkur Lekarski"},{"key":"R2-18-20241212","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/35001095","article-title":"Shiga-like toxins are neutralized by tailored multivalent carbohydrate ligands","volume":"403","author":"Kitov","year":"2000","journal-title":"Nature"},{"key":"R3-18-20241212","doi-asserted-by":"crossref","first-page":"5334","DOI":"10.1128\/AEM.65.12.5334-5337.1999","article-title":"Agar underlay method for recovery of sublethally heat-injured bacteria","volume":"65","author":"Kang","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"R4-18-20241212","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1111\/j.1365-2958.1989.tb00158.x","article-title":"Multi-gene amplification: Simultaneous detection of three virulence genes in diarrhoeal stool","volume":"3","author":"Frankel","year":"1989","journal-title":"Mol Microbiol"},{"key":"R6-18-20241212","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3109\/10799898009044098","article-title":"Interaction of Shigella Shigae cytotoxin with receptors on sensitive and insensitive cells","volume":"1","author":"Eiklid","year":"1980","journal-title":"J Recept Res"},{"key":"R7-18-20241212","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1128\/jcm.12.3.361-366.1980","article-title":"Quantitative microtiter cytotoxicity assay for Shigella toxin","volume":"12","author":"Gentry","year":"1980","journal-title":"J Clin Microbiol"},{"key":"R8-18-20241212","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/S0021-9258(19)86295-6","article-title":"Isolation and characterization of Shigella Shigae cytotoxin","volume":"255","author":"Olsnes","year":"1980","journal-title":"J Biol Chem"},{"key":"R9-18-20241212","doi-asserted-by":"crossref","first-page":"161","DOI":"10.2307\/1590381","article-title":"Rapid serological profiling by enzyme-linked immunosorbent assay. I. Measurement of antibody activity titer against Newcastle disease virus in a single serum dilution","volume":"27","author":"Snyder","year":"1983","journal-title":"Avian Dis"},{"key":"R10-18-20241212","doi-asserted-by":"crossref","first-page":"8094","DOI":"10.1016\/j.vaccine.2011.08.040","article-title":"Recombinant Shiga toxin B subunit elicits protection against Shiga toxin via mixed Th type immune response in mice","volume":"29","author":"Gupta","year":"2011","journal-title":"Vaccine"},{"key":"R11-18-20241212","doi-asserted-by":"crossref","first-page":"774","DOI":"10.2174\/092986610791190291","article-title":"Antibodies against recombinant Shiga toxin subunit B neutralize Shiga toxin toxicity in HeLa cells","volume":"17","author":"Gupta","year":"2010","journal-title":"Protein Pept Lett"},{"key":"R12-18-20241212","doi-asserted-by":"crossref","first-page":"8739","DOI":"10.1016\/S0021-9258(19)68906-4","article-title":"The cytotoxic activity of Shigella toxin. Evidence for catalytic inactivation of the 60 S ribosomal subunit","volume":"256","author":"Reisbig","year":"1981","journal-title":"J Biol Chem"},{"key":"R13-18-20241212","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1128\/CVI.00342-06","article-title":"Development of a simple latex agglutination assay for detection of Shiga toxin-producing Escherichia coli (STEC) by using polyclonal antibody against STEC","volume":"14","author":"Hajra","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"R14-18-20241212","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/jcm.25.1.115-118.1987","article-title":"Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins","volume":"25","author":"Kongmuang","year":"1987","journal-title":"J Clin Microbiol"},{"key":"R15-18-20241212","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/iai.50.3.695-700.1985","article-title":"Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli","volume":"50","author":"Strockbine","year":"1985","journal-title":"Infect Immun"},{"key":"R16-18-20241212","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.3390\/toxins7041163","article-title":"Detection of Shiga toxins by lateral flow assay","volume":"7","author":"Ching","year":"2015","journal-title":"Toxins (Basel"}],"container-title":["Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/02223309-201949030-00018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T23:01:24Z","timestamp":1751497284000},"score":1,"resource":{"primary":{"URL":"https:\/\/ijmr.org.in\/immunological-detection-assays-for-recombinant-shiga-toxin-shigella-dysenteriae\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.4103\/ijmr.ijmr_308_17","relation":{},"ISSN":["0971-5916"],"issn-type":[{"value":"0971-5916","type":"print"}],"subject":[],"published":{"date-parts":[[2019,3]]}}}