{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:10:17Z","timestamp":1766869817157,"version":"3.41.0"},"reference-count":29,"publisher":"Scientific Scholar","issue":"Suppl 1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,7]]},"abstract":"\n Background & objectives:<\/jats:title>\n \u03b2-lactamases play a predominant role in drug-resistance amongst Enterobacteriaceae<\/jats:italic>. Presence of genes on transferable plasmids encoding these enzymes favours their dissemination across species and genera within and outside geographical boundaries. This study was aimed to understand the presence of \u03b2-lactamases and transferable plasmids in clinical isolates of Klebsiella<\/jats:italic> spp. which can contribute to the spread of resistance determinants.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n A total of 41 clinical isolates of Klebsiella<\/jats:italic> spp., collected from a tertiary care centre in Kerala, India, were checked for antibiotic sensitivity and the presence of plasmids. The ability to produce extended-spectrum \u03b2-lactamases (ESBLs) and metallo-\u03b2-lactamases (MBLs) was screened for and confirmed in 29 plasmid-harbouring isolates. bla<\/jats:italic>\n NDM-1<\/jats:sub>-specific primers were used for polymerase chain reaction amplification with plasmid DNA as template to determine episomal prevalence of this gene and its sequence-based phylogeny employing similar sequences from GenBank. Plasmid replicon typing was also carried out to determine the presence of transferable plasmids.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Our results showed a high degree of multidrug-resistant (MDR) pathogens with ESBL production confirmed in 52 per cent, MBL in 31 per cent and co-production of both enzymes in seven per cent of the plasmid-bearing isolates. Plasmid DNA from 14 per cent of the isolates produced bla<\/jats:italic>\n NDM-1<\/jats:sub>-specific amplicons which showed sequence homology with those from bacteria of different genera and geographical areas. The predominant replicon type was found to be that of conjugative plasmids belonging to the incompatibility group - IncFIIK<\/jats:sub>.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n This study provides insight into the predominance of various \u03b2-lactamases and potent gene-disseminating agents in Klebsiella<\/jats:italic> spp. and emphasizes the need for constant surveillance of these pathogens to determine appropriate treatment strategies.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_31_16","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T05:53:27Z","timestamp":1513922007000},"page":"S38-S45","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of beta-lactamases, bla NDM-1 phylogeny & plasmid replicons in multidrug-resistant Klebsiella spp. from a tertiary care centre in south India"],"prefix":"10.25259","volume":"146","author":[{"given":"N.","family":"Nithya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabindran","family":"Remitha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. R.","family":"Jayasree","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Faisal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. R.","family":"Manish Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R1-6-20241212","doi-asserted-by":"crossref","first-page":"243","DOI":"10.5694\/mja14.01257","article-title":"Facing the challenge of multidrug-resistant Gram-negative bacilli in Australia","volume":"202","author":"Harris","year":"2015","journal-title":"Med J Aust"},{"key":"R2-6-20241212","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.jhin.2015.01.008","article-title":"Multidrug-resistant Gram-negative bacteria: A product of globalization","volume":"89","author":"Hawkey","year":"2015","journal-title":"J Hosp Infect"},{"key":"R3-6-20241212","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1517\/14656566.2014.914172","article-title":"Multidrug-resistant and extensively drug-resistant Gram-negative pathogens: Current and emerging therapeutic approaches","volume":"15","author":"Karaiskos","year":"2014","journal-title":"Expert Opin Pharmacother"},{"key":"R4-6-20241212","first-page":"211","article-title":"Prevalence of antimicrobial drug resistance of Klebsiella pneumoniae in India","volume":"1","author":"Sikarwar","year":"2011","journal-title":"Int J Biosci Biochem Bioinforma"},{"key":"R5-6-20241212","first-page":"907","article-title":"A ten year analysis of multi-drug resistant blood stream infections caused by Escherichia coli and Klebsiella pneumoniae in a tertiary care hospital","volume":"135","author":"Datta","year":"2012","journal-title":"Indian J Med Res"},{"key":"R6-6-20241212","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1128\/AAC.00585-11","article-title":"Genetic features of bla NDM-1-positive Enterobacteriaceae","volume":"55","author":"Poirel","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"R7-6-20241212","first-page":"70","article-title":"ESBL, MBL and Ampc b lactamases producing superbugs \u2013Havoc in the Intensive Care Units of Punjab India","volume":"7","author":"Oberoi","year":"2013","journal-title":"J Clin Diagn Res"},{"key":"R8-6-20241212","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1111\/1469-0691.12697","article-title":"Treating infections caused by carbapenemase-producing Enterobacteriaceae","volume":"20","author":"Tzouvelekis","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"R9-6-20241212","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3201\/EID2003.121157","article-title":"Surveillance for antimicrobial drug resistance in under-resourced countries","volume":"20","author":"Vernet","year":"2014","journal-title":"Emerg Infect Dis"},{"key":"R10-6-20241212","doi-asserted-by":"crossref","first-page":"e34752","DOI":"10.1371\/journal.pone.0034752","article-title":"Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131","volume":"7","author":"Bonnin","year":"2012","journal-title":"PLoS One"},{"key":"R11-6-20241212","doi-asserted-by":"crossref","first-page":"5046","DOI":"10.1128\/AAC.00774-09","article-title":"Characterization of a new metallo-\u03b2-lactamase gene bla NDM-1 and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India","volume":"53","author":"Yong","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"R12-6-20241212","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1111\/j.1469-0691.2010.03385.x","article-title":"New Delhi metallo-beta-lactamase (NDM-1): Towards a new pandemia?","volume":"16","author":"Rolain","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"R13-6-20241212","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4103\/0973-1229.77446","article-title":"Science, names giving and names calling: Change NDM-1 to PCM","volume":"9","author":"Singh","year":"2011","journal-title":"Mens Sana Monogr"},{"key":"R14-6-20241212","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1093\/jac\/dkq347","article-title":"Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants","volume":"65","author":"Villa","year":"2010","journal-title":"J Antimicrob Chemother"},{"key":"R15-6-20241212","doi-asserted-by":"crossref","first-page":"870","DOI":"10.3201\/eid1906.121515","article-title":"New Delhi metallo-b-lactamase-producing Enterobacteriaceae United States","volume":"19","author":"Rasheed","year":"2013","journal-title":"Emerg Infect Dis"},{"key":"R16-6-20241212","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1099\/mic.0.075762-0","article-title":"Prevalence of IncI1-I\u03b3and IncFIA-FIB type plasmids in extended-spectrum \u00df-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital","volume":"160","author":"Ali","year":"2014","journal-title":"Microbiology"},{"key":"R18-6-20241212","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disk method","volume":"45","author":"Bauer","year":"1966","journal-title":"Am J Clin Pathol"},{"key":"R20-6-20241212","doi-asserted-by":"crossref","first-page":"302","DOI":"10.4103\/0255-0857.83918","article-title":"Metallo beta lactamase producing Pseudomonas aeruginosa and Acinetobacter baumannii","volume":"29","author":"John","year":"2011","journal-title":"Indian J Med Microbiol"},{"key":"R21-6-20241212","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"Poirel","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R22-6-20241212","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol Biol Evol"},{"key":"R23-6-20241212","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1128\/AAC.01707-08","article-title":"Resistance plasmid families in Enterobacteriaceae","volume":"53","author":"Carattoli","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"R24-6-20241212","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.5455\/ijmsph.2016.20102015181","article-title":"Occurrence of various beta-lactamase enzyme-producing Enterobacteriaceae in the hospital effluent: A wake-up call","volume":"5","author":"Devaraju","year":"2016","journal-title":"Int J Med Sci Public Health"},{"key":"R25-6-20241212","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S1473-3099(10)70143-2","article-title":"Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: A molecular, biological, and epidemiological study","volume":"10","author":"Kumarasamy","year":"2010","journal-title":"Lancet Infect Dis"},{"key":"R26-6-20241212","doi-asserted-by":"crossref","first-page":"e01550","DOI":"10.1128\/genomeA.01550-15","article-title":"Complete genome sequence of Klebsiella pneumoniae subsp. pneumoniae KP617, coproducing OXA-232 and NDM-1 carbapenemases, isolated in South Korea","volume":"4","author":"Kwon","year":"2016","journal-title":"Genome Announc"},{"key":"R27-6-20241212","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.nmni.2015.06.002","article-title":"High prevalence of bla OXA-23 in Acinetobacter spp. and detection of bla NDM-1 in A. soli in Cuba: Report from National Surveillance Program (2010-2012)","volume":"7","author":"Qui\u00f1ones","year":"2015","journal-title":"New Microbes New Infect"},{"key":"R29-6-20241212","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1128\/AAC.02773-13","article-title":"Complete nucleotide sequence of two multidrug-resistant IncR plasmids from Klebsiella pneumoniae","volume":"58","author":"Compain","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"R30-6-20241212","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1038\/emi.2012.37","article-title":"Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China","volume":"1","author":"Ho","year":"2012","journal-title":"Emerg Microbes Infect"},{"key":"R31-6-20241212","doi-asserted-by":"crossref","first-page":"2487","DOI":"10.1093\/jac\/dkt237","article-title":"Multiclonal dispersal of KPC genes following the emergence of non-ST258 KPC-producing Klebsiella pneumoniae clones in Madrid, Spain","volume":"68","author":"Ruiz-Garbajosa","year":"2013","journal-title":"J Antimicrob Chemother"},{"key":"R32-6-20241212","doi-asserted-by":"crossref","first-page":"3898","DOI":"10.1128\/AEM.00522-13","article-title":"Molecular characterization of CTX-M b-lactamase and associated addiction systems in Escherichia coli circulating among cattle, farm workers, and the farm environment","volume":"79","author":"Tamang","year":"2013","journal-title":"Appl Environ Microbiol"}],"container-title":["Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/02223309-201746001-00006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T22:59:43Z","timestamp":1751497183000},"score":1,"resource":{"primary":{"URL":"https:\/\/ijmr.org.in\/analysis-of-beta-lactamases-blandm-1-phylogeny-plasmid-replicons-in-multidrug-resistant-klebsiella-spp-from-a-tertiary-care-centre-in-south-india\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":29,"journal-issue":{"issue":"Suppl 1","published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.4103\/ijmr.ijmr_31_16","relation":{},"ISSN":["0971-5916"],"issn-type":[{"type":"print","value":"0971-5916"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}