{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:07:40Z","timestamp":1773493660106,"version":"3.50.1"},"reference-count":18,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,8]]},"abstract":"\n Background & objectives:<\/jats:title>\n Despite being a tropical country, vitamin D deficiency is highly prevalent in India with studies indicating 40-99 per cent prevalence. Apart from calcium and phosphate metabolism, vitamin D is involved in cell cycle regulation, cardiovascular, hepatoprotection. The metabolism of vitamin D is regulated by vitamin D tool genes (CYP2R1\/CYP27B1\/CYP24A1\/VDR<\/jats:italic>). The promoter regions of some of these genes have CpG islands, making them prone to methylation induced gene silencing, which may cause a reduction in circulating vitamin D levels. Epigenetic basis of vitamin D deficiency is yet to be studied in India, and hence, this pilot study was aimed to analyze whether methylation levels of CYP2R1<\/jats:italic> gene were correlated with the levels of 25(OH)D in healthy, adult individuals in Indian population.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n In this cross-sectional study, healthy adults of 18-45 yr of age with no history of malabsorption, thyroidectomy, chronic illness or therapeutic vitamin D supplementation were recruited. DNA methylation analysis was carried out by methylation specific quantitative PCR. Serum calcium, phosphate and vitamin D levels were also quantified. Statistical analysis was done by R 4.0.5 software.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n A total of 61 apparently healthy adults were analyzed. The serum vitamin D levels did not correlate with CYP2R1<\/jats:italic> methylation levels in our study population. Significant positive correlation was observed between age and serum vitamin D levels. Significant association of gender was found with CYP2R1<\/jats:italic> methylation levels.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n This study found no significant correlation between levels of CYP2R1<\/jats:italic> methylation and circulating 25(OH)D deficiency. Further studies on the Indian population having a larger sample size including entire vitamin D tool genes, among different ethnic groups may be conducted to elucidate molecular etiology of circulating 25(OH)D deficiency. The high prevalence of normal serum calcium and phosphate levels among vitamin D deficient subjects in this study coupled with the strikingly high prevalence of the deficiency at the national level, may suggest the need to revise the cut-off criteria for vitamin D deficiency in the Indian population.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_3493_21","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:47:46Z","timestamp":1693802866000},"page":"197-200","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Correlation of CYP2R1 gene promoter methylation with circulating vitamin D levels among healthy adults"],"prefix":"10.25259","volume":"158","author":[{"given":"Ashvin","family":"Varadharajan","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.K.","family":"Sibin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.V.","family":"Athira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arijit Kumar","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Army Institute of Cardio Thoracic Sciences, Pune, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pratibha","family":"Misra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, 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