{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:19:04Z","timestamp":1792671544086,"version":"4.5.0"},"reference-count":16,"publisher":"Scientific Scholar","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,6]]},"abstract":"\n Background & objectives:<\/jats:title>\n The diagnosis of scrub typhus (ST) is usually done using enzyme-linked immunosorbent assay (ELISA) due to its ease of performance and reading objectivity. The cut-off value for ELISA needs to be calculated for each geographical location as it depends on zonal endemicity of the disease. This study was, therefore, undertaken to calculate the pan-India cut-off for anti-Orientia tsutsugamushi<\/jats:italic> (OT) immunoglobulin M (IgM) by ELISA.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Samples from cases (cases of ST) and controls (voluntary, consenting, healthy adults) were collected by a network of 29 laboratories across India and tested for anti-OT IgM by immunofluorescence assay (IFA), the considered gold standard test. These samples were retested by ELISA for anti-OT IgM and their optical densities (ODs) were used for cut-off estimation by receiver operating characteristic (ROC) curve.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Anti-OT IgM ELISA ODs from 273 controls and 136 cases were used for the cut-off estimation. The ODs of the anti-OT IgM ELISA on healthy individuals and those of confirmed ST cases ranged from 0.1 to 0.75 and 0.5 to 4.718, respectively. ROC curve-based cut-off for ELISA was calculated as 0.554 at a sensitivity of 95.2 per cent and specificity of 95.1 per cent. A value of >1 was noted to have a specificity of 100 per cent in diagnosing ST.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The cut-off calculated for India was similar to the previous cut-off that was used until now.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_4098_20","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T07:10:31Z","timestamp":1713251431000},"page":"519-523","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":12,"title":["Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M"],"prefix":"10.25259","volume":"157","author":[{"given":"Amita","family":"Jain","sequence":"first","affiliation":[{"name":"Department of Microbiology, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parul","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Microbiology, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay C.","family":"Rebello","sequence":"additional","affiliation":[{"name":"Department of Microbiology, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moulika","family":"Todaria","sequence":"additional","affiliation":[{"name":"Department of Microbiology, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harmanmeet","family":"Kaur","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, 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