{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:22:37Z","timestamp":1751516557814,"version":"3.41.0"},"reference-count":31,"publisher":"Scientific Scholar","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5]]},"abstract":"\n Background & objectives:<\/jats:title>\n To examine \u03b2-D-mannuronic acid (M2000) effects on L-selectin shedding and leucocyte function-associated antigen-1 (LFA-1) expression as mechanisms of action of this drug in patients with ankylosing spondylitis (AS).<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n To investigate the molecular consequences of \u03b2-D-mannuronic acid on L-selectin shedding, flow cytometry method was used. Furthermore, the effect of it on LFA-1 gene expression was analyzed by using quantitative real time (qRT)-PCR technique.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n The LFA-1 expression in patients with AS was higher than controls (P<\/jats:italic>=0.046). The LFA-1 expression after 12 wk therapy with \u03b2-D-mannuronic acid was meaningfully decreased (P<\/jats:italic>=0.01). After 12 wk treatment with \u03b2-D-mannuronic acid, the frequency of CD62L-expressing CD4+ T cells in patients with AS, was not considerably altered, compared to the patients before therapy (P<\/jats:italic>=0.5). Furthermore, after 12 wk therapy with \u03b2-D-mannuronic acid, L-selectin expression levels on CD4+ T-cells in patients with AS, were not remarkably changed, compared to the expression levels of these in patients before treatment (P<\/jats:italic>=0.2).<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The results of this study for the first time showed that \u03b2-D-mannuronic acid can affect events of adhesion cascade in patients with AS. Moreover, \u03b2-D-mannuronic acid presented as an acceptable benefit to AS patients and could aid in the process of disease management.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_448_19","type":"journal-article","created":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T08:25:39Z","timestamp":1699518339000},"page":"453-459","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of cell adhesion molecules (LFA-1 and L-selectin) in ankylosing spondylitis patients after treatment with \u03b2-D-mannuronic acid (M2000)"],"prefix":"10.25259","volume":"157","author":[{"given":"Mohammad Javad","family":"Fattahi","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran"},{"name":"Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, 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