{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:07:37Z","timestamp":1788984457640,"version":"build-2803163510"},"reference-count":22,"publisher":"Scientific Scholar","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,5]]},"abstract":"\n Background & objectives:<\/jats:title>\n Individual donation nucleic acid testing (ID-NAT) is considered as sensitive technology to assess blood safety from viral transfusion-transmissible infections (TTIs) in blood donors. The present study was aimed to analyze the results of ID-NAT for three years (2013-2015) with special reference to different types of donors and their age ranges in a tertiary care centre in north India.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n The results of ID-NAT for three years were retrospectively analyzed at our centre. A total of 168,433 donations were tested with ID-NAT, of which 10,467 were tested with Procleix\u00ae<\/jats:sup> Ultrio\u00ae<\/jats:sup> reagents and 157,966 were tested with Procleix\u00ae<\/jats:sup> UltrioPlus\u00ae<\/jats:sup> reagents, and the results were compared with those of serology to calculate the NAT yield in voluntary, replacement, first-time and repeat donors.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n A combined NAT yield was observed as one in 1031 out of 167,069 seronegative donations with HBV yield as one in 1465, HCV yield as one in 3885 and HIV-1 as one in 167,069. Yield for co-infection (HCV and HBV) was one in 41,767. A high NAT yield was observed in replacement donors (1 in 498) as compared to voluntary donors (1 in 1320).<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n Addition of NAT to serology improved the blood safety in our centre interdicting possibility of 150 TTIs annually. It has also reemphasized the safety of voluntary over replacement donors. The results also highlight the need of proper counselling, notification and referral guidelines of NAT yield donors in our country and other countries which lack them.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmr.ijmr_822_17","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T07:29:56Z","timestamp":1566372596000},"page":"633-640","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Initial trends of individual donation nucleic acid testing in voluntary & replacement donors from a tertiary care centre in north India"],"prefix":"10.25259","volume":"149","author":[{"given":"Rekha","family":"Hans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Marwaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suchet","family":"Sachdev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratti Ram","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","reference":[{"key":"R1-10-20241212","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1111\/j.1537-2995.2008.01718.x","article-title":"Experience of German Red Cross blood donor services with nucleic acid testing: Results of screening more than 30 million blood donations for human immunodeficiency virus-1, hepatitis C virus, and hepatitis B virus","volume":"48","author":"Hourfar","year":"2008","journal-title":"Transfusion"},{"key":"R2-10-20241212","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/0973-6247.126679","article-title":"Nucleic acid testing-benefits and constraints","volume":"8","author":"Hans","year":"2014","journal-title":"Asian J Transfus Sci"},{"key":"R8-10-20241212","first-page":"140","article-title":"Multicenter evaluation of individual donor nucleic acid testing (NAT) for simultaneous detection of human immunodeficiency virus -1 & hepatitis B & C viruses in Indian blood donors","volume":"127","author":"Makroo","year":"2008","journal-title":"Indian J Med Res"},{"key":"R9-10-20241212","doi-asserted-by":"crossref","first-page":"718671","DOI":"10.5402\/2012\/718671","article-title":"Need for nucleic acid testing in countries with high prevalence of transfusion-transmitted infections","volume":"2012","author":"Jain","year":"2012","journal-title":"ISRN Hematol"},{"key":"R10-10-20241212","first-page":"207","article-title":"Individual donor nucleic acid testing for blood safety against HIV-1 and hepatitis B and C viruses in a tertiary care hospital","volume":"25","author":"Chatterjee","year":"2012","journal-title":"Natl Med J India"},{"key":"R11-10-20241212","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.transci.2013.03.004","article-title":"Nucleic acid testing for blood banks: An experience from a tertiary care centre in New Delhi, India","volume":"49","author":"Agarwal","year":"2013","journal-title":"Transfus Apher Sci"},{"key":"R13-10-20241212","doi-asserted-by":"crossref","first-page":"313","DOI":"10.4103\/IJPM.IJPM_361_16","article-title":"Nucleic acid amplification testing in Indian blood banks: A review with perspectives","volume":"60","author":"Ghosh","year":"2017","journal-title":"Indian J Pathol Microbiol"},{"key":"R14-10-20241212","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/j.1348-0421.2001.tb01300.x","article-title":"The first large-scale nucleic acid amplification testing (NAT) of donated blood using multiplex reagent for simultaneous detection of HBV, HCV, and HIV-1 and significance of NAT for HBV","volume":"45","author":"Ohnuma","year":"2001","journal-title":"Microbiol Immunol"},{"key":"R15-10-20241212","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1111\/j.1537-2995.2010.02622.x","article-title":"Prevalence, incidence, and residual risk of human immunodeficiency virus and hepatitis C virus infections among United States blood donors since the introduction of nucleic acid testing","volume":"50","author":"Zou","year":"2010","journal-title":"Transfusion"},{"key":"R16-10-20241212","first-page":"s58","article-title":"The results of nucleic acid testing in remunerated and non-remunerated blood donors in Lithuania","volume":"12","author":"Kalibatas","year":"2014","journal-title":"Blood Transfus"},{"key":"R17-10-20241212","first-page":"23","article-title":"The impact of different HCV blood screening technologies on the reduction of transfusion transmitted HCV infection risk in Egypt","volume":"96","author":"El Ekiaby","year":"2009","journal-title":"Vox Sang"},{"key":"R18-10-20241212","first-page":"172","article-title":"A pilot study on screening blood donors with individual-donation nucleic acid testing in China","volume":"12","author":"Dong","year":"2014","journal-title":"Blood Transfus"},{"key":"R19-10-20241212","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1111\/trf.13017","article-title":"Impact of nucleic acid amplification test on screening of blood donors in Northern Pakistan","volume":"55","author":"Niazi","year":"2015","journal-title":"Transfusion"},{"key":"R20-10-20241212","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4103\/0973-6247.150938","article-title":"Automated nucleic acid amplification testing in blood banks: An additional layer of blood safety","volume":"9","author":"Chigurupati","year":"2015","journal-title":"Asian J Transfus Sci"},{"key":"R21-10-20241212","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4103\/0973-6247.115566","article-title":"Transfusion transmittable infections \u2013 Seroprevalence among blood donors in a tertiary care hospital of Delhi","volume":"7","author":"Pathak","year":"2013","journal-title":"Asian J Transfus Sci"},{"key":"R22-10-20241212","doi-asserted-by":"crossref","first-page":"79","DOI":"10.7883\/yoken.JJID.2008.79","article-title":"Prevalence of human immunodeficiency virus (HIV), hepatitis B virus, and hepatitis C virus in three groups of populations at high risk of HIV infection in Amritsar (Punjab), Northern India","volume":"61","author":"Jindal","year":"2008","journal-title":"Jpn J Infect Dis"},{"key":"R23-10-20241212","doi-asserted-by":"crossref","first-page":"91","DOI":"10.4103\/2348-3334.134268","article-title":"Epidemiological profile of seropositive blood donors at a tertiary care hospital in North India","volume":"2","author":"Koshy","year":"2014","journal-title":"CHRISMED J Health Res"},{"key":"R24-10-20241212","first-page":"00046","article-title":"Hepatitis B virus (HBV) and s-escape mutants: From the beginning until now","volume":"2","author":"Perazzo","year":"2015","journal-title":"J Hum Virol Retrovirol"},{"key":"R26-10-20241212","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4103\/0973-6247.98927","article-title":"True positivity of anti-hepatitis C virus enzyme-linked immunosorbent assay reactive blood donors: A prospective study done in Western India","volume":"6","author":"Tulsiani","year":"2012","journal-title":"Asian J Transfus Sci"},{"key":"R27-10-20241212","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/j.1537-2995.2005.00473.x","article-title":"Lack of correlation between hepatitis B surface antigen and hepatitis B virus DNA levels in blood donors","volume":"45","author":"Allain","year":"2005","journal-title":"Transfusion"},{"key":"R28-10-20241212","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/voxs.12257","article-title":"Five years of experience with ID-NAT at a tertiary care centre in North India: An interdictory step in preventing the transfusion-transmitted infections","volume":"11","author":"Kabita","year":"2016","journal-title":"ISBT Sci Ser"},{"key":"R29-10-20241212","doi-asserted-by":"crossref","first-page":"412105","DOI":"10.1155\/2014\/412105","article-title":"Screening donated blood for transfusion transmitted infections by serology along with NAT and response rate to notification of reactive results: An Indian experience","volume":"2014","author":"Chaurasia","year":"2014","journal-title":"J Blood Transfus"}],"container-title":["Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/02223309-201949050-00010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T23:01:43Z","timestamp":1751497303000},"score":1,"resource":{"primary":{"URL":"https:\/\/ijmr.org.in\/initial-trends-of-individual-donation-nucleic-acid-testing-in-voluntary-replacement-donors-from-a-tertiary-care-centre-in-north-india\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.4103\/ijmr.ijmr_822_17","relation":{},"ISSN":["0971-5916"],"issn-type":[{"value":"0971-5916","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]}}}