{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:22:04Z","timestamp":1787775724652,"version":"build-2784847793"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Nontuberculous mycobacteria (NTM) infections are an emerging global health concern with increasing incidence. Conventional identification methods for NTM species in clinical settings are prone to errors. This study evaluates a newer method, polymerase chain reaction-restriction enzyme analysis (PCR-REA) of the\n                      <jats:italic toggle=\"yes\">rpo<\/jats:italic>\n                      B gene, for NTM species identification. The study identified NTM species in clinical samples using conventional biochemical techniques and compared the results with PCR-REA of the\n                      <jats:italic toggle=\"yes\">rpo<\/jats:italic>\n                      B gene. This cross-sectional study was conducted at a tertiary health-care center in North India over 18 months, analyzing both pulmonary and extrapulmonary samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Two hundred and forty-seven NTM isolates were identified using phenotypic and biochemical methods. The same isolates were subjected to\n                      <jats:italic toggle=\"yes\">rpo<\/jats:italic>\n                      B gene amplification by PCR followed by REA using Msp I and Hae III enzymes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Conventional methods identified 12 different NTM species (153 slow-growing and 94 rapid-growing), whereas PCR-REA identified 16 species (140 slow-growing, 107 rapid-growing). The\n                      <jats:italic toggle=\"yes\">Mycobacterium avium<\/jats:italic>\n                      intracellulare complex was the most common species isolated. PCR-REA demonstrated higher resolution in species identification, particularly in differentiating within species complexes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      PCR-REA of the\n                      <jats:italic toggle=\"yes\">rpo<\/jats:italic>\n                      B gene proves to be a simple, rapid, and more discriminative tool for NTM species identification compared to conventional methods. This technique could significantly improve the diagnosis and management of emerging NTM infections in clinical settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_134_24","type":"journal-article","created":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T16:00:37Z","timestamp":1726416037000},"page":"307-313","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Nontuberculous Mycobacterium Species by Polymerase Chain Reaction - Restriction Enzyme Analysis (PCR-REA) of rpoB gene in Clinical Isolates"],"prefix":"10.4103","volume":"13","author":[{"given":"Raj Narayan","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yellanki Yashwanth","family":"Chowdary","sequence":"additional","affiliation":[{"name":"Department of Medicine, Shri B. 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