{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:22:00Z","timestamp":1787775720885,"version":"build-2784847793"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Tuberculosis (TB) is caused due to the infection of\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      (MTB) and it can infect the various parts of the human body. The disease is highly prevalent and is the second most common cause of death worldwide after COVID-19. Apart from sputum specimen, it is exceedingly difficult to diagnose due to its paucibacillary nature. The current study was intended to evaluate the accuracy of Smart Sure\u2122 MTB and multidrug-resistant-TB (MDR-TB) kits (Genetix Biotech Asia Pvt. Ltd., India) with Xpert ultra and Mycobacterium growth indicator tube (MGIT) culture on nonsputum specimens from TB suspects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A total of 205 nonsputum specimens were received between October 2023 and May 2024 at Intermediate Reference Laboratory, Department of Medicine, All India Institute of Medical Sciences, New Delhi, India. Xpert ultra and Smart Sure\u2122 MTB and MDR-TB tests were done directly on samples. However, processed specimens were used for MGIT culture and drug-susceptibility testing (DST). Invalid and MGIT contaminated specimens were excluded from the final calculation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Overall, sensitivity and specificity of Smart Sure\u2122 MTB screening kit was 71.59% and 98.28%, respectively, with Xpert ultra and 68.35% and 90.83%, respectively, with MGIT culture. While comparing with both Xpert ultra and MGIT-DST to detect rifampicin (RIF) resistant, Smart Sure\u2122 MDR-TB kits showed sensitivity of 75.0% and 100% of specificity. However, for isoniazid (INH) resistance, Smart Sure\u2122 MDR-TB kits showed 100% of sensitivity and specificity with MGIT-DST.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>For the detection of MTB and its drug-resistance patterns (RIF and INH) in the specimens other than sputum, Smart Sure\u2122 MTB and MDR-TB kits could play a vital role in TB endemic countries. While comparing the set-ups and skilled staffs, it required almost same as compared with previously approved WHO diagnostics used in resource-limited countries.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_140_24","type":"journal-article","created":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T16:00:52Z","timestamp":1726416052000},"page":"275-281","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostics Evaluation of Smart Sure\u2122 Mycobacterium tuberculosis Screening Kit and Smart Sure\u2122 Multidrug-resistant Tuberculosis Detection Kit on Nonsputum Specimens at a Tertiary Care Center of North India"],"prefix":"10.4103","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3722-4310","authenticated-orcid":false,"given":"Pankaj","family":"Jorwal","sequence":"first","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binit Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisha","family":"Khandelwal","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, School of Basic Sciences and Research, Sharda University, Grater Noida, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Payal","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Biotechnology, Chandigarh University, Mohali, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Nischal","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish","family":"Soneja","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prayas","family":"Sethi","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Dhawan","sequence":"additional","affiliation":[{"name":"Department of Public Health, SHARE, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveet","family":"Wig","sequence":"additional","affiliation":[{"name":"Department of Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,9,14]]},"reference":[{"key":"R1-20241212","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1038\/s41598-024-52130-3","article-title":"Usefulness of xpert MTB\/RIF ultra for rapid diagnosis of extrapulmonary tuberculosis in Tunisia","volume":"14","author":"Bouzouita","year":"2024","journal-title":"Sci Rep"},{"key":"R2-20241212","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.pulmoe.2023.05.007","article-title":"Clinical impact of Xpert\u00ae 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India\u201d","volume":"5","author":"Sharma","year":"2021","journal-title":"Acta Sci Med Sci"},{"key":"R10-20241212","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0167-7012(00)00160-3","article-title":"Development of polymerase chain reaction-based assays for bacterial gene detection","volume":"41","author":"Johnson","year":"2000","journal-title":"J Microbiol Methods"},{"key":"R14-20241212","article-title":"Global Laboratory Initiative, Mycobacteriology Laboratory Manual, Stop TB Partnership, Global TB Programme, WHO, Geneva, Switzerland, First Edition","year":"2014"},{"key":"R16-20241212","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1007\/s44197-023-00150-z","article-title":"Evaluation of Xpert MTB\/RIF ultra for the diagnosis of extrapulmonary tuberculosis: A retrospective analysis in Saudi Arabia","volume":"13","author":"Slail","year":"2023","journal-title":"J Epidemiol Glob Health"},{"key":"R17-20241212","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4103\/ijmy.ijmy_83_19","article-title":"Evaluation of GeneXpert Mycobacterium tuberculosis\/Rifampin for the detection of Mycobacterium tuberculosis complex and rifampicin resistance in nonrespiratory clinical specimens","volume":"8","author":"Habous","year":"2019","journal-title":"Int J Mycobacteriol"},{"key":"R18-20241212","doi-asserted-by":"crossref","first-page":"47","DOI":"10.4103\/ijmy.ijmy_199_23","article-title":"Correlation of cyclic threshold values generated by GeneXpert Ultra MTB\/RIF and fluorescence microscopy to predict mycobacterial burden in suspected cases of pulmonary tuberculosis","volume":"13","author":"Gota","year":"2024","journal-title":"Int J Mycobacteriol"},{"key":"R20-20241212","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4103\/ijmy.ijmy_86_23","article-title":"Comparison of Xpert MTB\/RIF assay, line probe assay, and culture in diagnosis of pulmonary tuberculosis on bronchoscopic specimen","volume":"12","author":"Kanade","year":"2023","journal-title":"Int J Mycobacteriol"}],"container-title":["The International Journal of 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History"}},{"value":"2024-08-20","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}