{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:52:19Z","timestamp":1791305539894,"version":"4.3.1"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Rapid detection of isoniazid (INH) resistance remains important for tuberculosis management, although targeted molecular assays may not capture the full genetic heterogeneity underlying phenotypic resistance. This study evaluated multiplex allele-specific polymerase chain reaction (MAS-PCR) for detecting INH resistance-associated molecular patterns in\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>This cross-sectional diagnostic accuracy study included 100 sputum specimens, comprising 70 acid-fast bacilli smear-positive and 30 smear-negative specimens. Phenotypic INH susceptibility was determined using Mycobacteria Growth Indicator Tube drug-susceptibility testing (DST-MGIT). MAS-PCR interrogated resistance-associated regions, with particular emphasis on katG315 and ahpC. Diagnostic performance and agreement were assessed in the primary evaluable population of 64 smear-positive specimens with paired MAS-PCR and DST-MGIT results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Among the 64 evaluable specimens, MAS-PCR yielded 5 true-positive, 3 false-negative, 0 false-positive, and 56 true-negative results. Sensitivity was 62.5% (95% confidence interval [CI]: 24.5%\u201391.5%), specificity of 100.0% (95% CI: 93.6%\u2013100.0%), positive predictive value of 100.0% (95% CI: 47.8%\u2013100.0%), negative predictive value of 94.9% (95% CI: 85.9%\u201398.9%), and accuracy of 95.3% (95% CI: 86.9%\u201399.0%). Overall agreement was 95.3%, with substantial agreement beyond chance (Cohen\u2019s \u03ba\u22480.745). The exact McNemar\u2019s test showed no evidence of systematic directional discordance (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.250). The targeted katG315 mutation-associated pattern occurred exclusively among phenotypically resistant specimens, whereas no targeted ahpC pattern was detected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>MAS-PCR demonstrated high specificity and substantial agreement with DST-MGIT among evaluable smear-positive specimens but incomplete sensitivity for phenotypic INH resistance. These findings support its use as a complementary targeted molecular approach rather than a replacement for phenotypic DST.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_162_26","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:00:06Z","timestamp":1790823606000},"page":"311-322","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Performance of Multiplex Allele-specific Polymerase Chain Reaction for Isoniazid Resistance in Mycobacterium tuberculosis: Comparison with Phenotypic Mycobacteria Growth Indicator Tube Drug-susceptibility Testing"],"prefix":"10.4103","volume":"15","author":[{"family":"Subair","sequence":"first","affiliation":[{"name":"Department of Medical Technology Laboratory, Faculty of Science and Technology, Megarezky University, Makassar, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainul","family":"Muttaqin","sequence":"additional","affiliation":[{"name":"Hasanuddin University Medical Research Center, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indas Wari","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Laboratory, Faculty of Science and Technology, Megarezky University, Makassar, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Nasrum","family":"Massi","sequence":"additional","affiliation":[{"name":"Hasanuddin University Medical Research Center, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia"},{"name":"Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1445-9579","authenticated-orcid":false,"family":"Handayani","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Laboratory, Faculty of Science and Technology, Megarezky University, Makassar, Indonesia"},{"name":"Hasanuddin University Medical Research Center, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"R3-20261006","doi-asserted-by":"crossref","first-page":"100379","DOI":"10.1016\/j.jctube.2023.100379","article-title":"Prevalence of Mycobacterium tuberculosis mutations associated with isoniazid and rifampicin resistance: A systematic review and meta-analysis","volume":"32","author":"Rostamian","year":"2023","journal-title":"J Clin Tuberc Other Mycobact Dis"},{"key":"R4-20261006","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/s41467-023-44325-5","article-title":"Quantitative measurement of antibiotic resistance in Mycobacterium tuberculosis reveals genetic determinants of resistance and susceptibility in a target gene approach","volume":"15","author":"Barilar","year":"2024","journal-title":"Nat Commun"},{"key":"R6-20261006","doi-asserted-by":"crossref","first-page":"206","DOI":"10.4103\/ijmy.ijmy_106_24","article-title":"Pharmacogenetic study of drugs affecting Mycobacterium tuberculosis","volume":"13","author":"Shabani","year":"2024","journal-title":"Int J Mycobacteriol"},{"key":"R8-20261006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2024.1372647","article-title":"Evaluation of a real-time PCR assay performance to detect Mycobacterium tuberculosis, rifampicin, and isoniazid resistance in sputum specimens: A multicenter study in two major cities of Indonesia","volume":"15","author":"Parwati","year":"2024","journal-title":"Front Microbiol"},{"key":"R9-20261006","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4103\/ijmy.ijmy_194_23","article-title":"Diagnostic performance of STANDARD\u2122 M10 multidrug-resistant tuberculosis assay for detection of Mycobacterium tuberculosis and rifampicin and isoniazid resistance in Zimbabwe","volume":"13","author":"Stephen","year":"2024","journal-title":"Int J Mycobacteriol"},{"key":"R10-20261006","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s12941-024-00741-z","article-title":"Detection of multidrug-resistance in Mycobacterium tuberculosis by phenotype- and molecular-based assays","volume":"23","author":"Vasiliauskait\u0117","year":"2024","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"R11-20261006","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4103\/ijmy.ijmy_37_24","article-title":"The role of efflux pumps transporter in multi-drug resistant tuberculosis: Mycobacterial memberane protein (MmpL5)","volume":"13","author":"Farnia","year":"2024","journal-title":"Int J Mycobacteriol"},{"key":"R12-20261006","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12929-021-00783-x","article-title":"Integrative utility of long read sequencing-based whole genome analysis and phenotypic assay on differentiating isoniazid-resistant signature of Mycobacterium tuberculosis","volume":"28","author":"Yu","year":"2021","journal-title":"J Biomed Sci"},{"key":"R13-20261006","doi-asserted-by":"crossref","first-page":"e0026525","DOI":"10.1128\/aac.00265-25","article-title":"The ahpC c-54t compensatory mutation is not always a valid surrogate for isoniazid resistance in Mycobacterium tuberculosis","volume":"69","author":"Sz\u00e9l","year":"2025","journal-title":"Antimicrob Agents Chemother"},{"key":"R14-20261006","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.ijtb.2023.03.019","article-title":"Detection of katG, inhA and ahpC gene mutation in clinical isolates of isoniazid-resistant Mycobacterium tuberculosis in Makassar City, South Sulawesi, Indonesia","volume":"71","author":"Junaedi","year":"2024","journal-title":"Indian J Tuberc"},{"key":"R15-20261006","doi-asserted-by":"crossref","first-page":"974428","DOI":"10.3389\/fmicb.2022.974428","article-title":"Clinical utility of target amplicon sequencing test for rapid diagnosis of drug-resistant Mycobacterium tuberculosis from respiratory specimens","volume":"13","author":"Leung","year":"2022","journal-title":"Front Microbiol"}],"container-title":["The International Journal of 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History"}},{"value":"2026-08-29","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}