{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:34:15Z","timestamp":1786649655564,"version":"build-2736575974"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,4]]},"abstract":"\n Background:<\/jats:title>\n \n Bovine tuberculosis (bTB) is a disease primarily caused by\n Mycobacterium bovis<\/jats:italic>\n . Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with\n M. bovis<\/jats:italic>\n . We compared the CFU counts from vaccinated and challenged mice with\n M. bovis<\/jats:italic>\n using data from a previous experiment.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n CFUs obtained from the lungs of vaccinated and\n M. bovis<\/jats:italic>\n -challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal\u2013Wallis, followed by a Dunn\u2019s multiple comparison test.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette\u2013Gu\u00e9rin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated\n M. bovis<\/jats:italic>\n triple mutant strain reached their final CFU counts only after 4 weeks of culturing.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_32_25","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T15:00:32Z","timestamp":1750431632000},"page":"140-144","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Dynamics of the In vitro Growing of Mycobacterium bovis from the Lungs of Vaccinated and Infected Mice"],"prefix":"10.4103","volume":"14","author":[{"given":"Federico Carlos","family":"Blanco","sequence":"first","affiliation":[{"name":"Institute of Agrobiotechnology and Molecular Biology (IABIMO), National Institute of Agricultural Technology (INTA)-National Scientific and Technical Research Council (CONICET), Hurlingham, Buenos Aires, Argentina"},{"name":"Institute of Biotechnology, Centre for Veterinary and Agronomics Research (CICVyA), INTA, Nicol\u00e1s Repetto and De los Reseros, Hurlingham, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura In\u00e9s","family":"Klepp","sequence":"additional","affiliation":[{"name":"Institute of Agrobiotechnology and Molecular Biology (IABIMO), National Institute of Agricultural Technology (INTA)-National Scientific and Technical Research Council (CONICET), Hurlingham, Buenos Aires, Argentina"},{"name":"Institute of Biotechnology, Centre for Veterinary and Agronomics Research (CICVyA), INTA, Nicol\u00e1s Repetto and De los Reseros, Hurlingham, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Lourdes","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Institute of Agrobiotechnology and Molecular Biology (IABIMO), National Institute of Agricultural Technology (INTA)-National Scientific and Technical Research Council (CONICET), Hurlingham, Buenos Aires, Argentina"},{"name":"Institute of Biotechnology, Centre for Veterinary and Agronomics Research (CICVyA), INTA, Nicol\u00e1s Repetto and De los Reseros, Hurlingham, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8637-7180","authenticated-orcid":false,"given":"Fabiana","family":"Bigi","sequence":"additional","affiliation":[{"name":"Institute of Agrobiotechnology and Molecular Biology (IABIMO), National Institute of Agricultural Technology (INTA)-National Scientific and Technical Research Council (CONICET), Hurlingham, Buenos Aires, Argentina"},{"name":"Institute of Biotechnology, Centre for Veterinary and Agronomics Research (CICVyA), INTA, 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