{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:24:32Z","timestamp":1787840672990,"version":"build-2784847793"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium lepare<\/jats:italic>\n                      -induced leprosy continues to pose a significant public health threat. Drug-resistant strains pose a major challenge for effective management, necessitating molecular studies to identify resistance-associated mutations and guide appropriate therapy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      A cross-sectional analysis of a total of 47 samples, including slit-skin smear and biopsy specimens, were collected along with relevant clinical details. Fifteen samples that tested positive for acid-fast bacilli were further processed. Amplification of\n                      <jats:italic toggle=\"yes\">folP1<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      genes was performed using polymerase chain reaction, followed by automated capillary sequencing to identify mutations associated with dapsone, rifampicin, and ofloxacin resistance, respectively, in\n                      <jats:italic toggle=\"yes\">M. leprae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Sequencing revealed no\n                      <jats:italic toggle=\"yes\">folP1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                      gene mutations in any of the 15 isolates sequenced in this study, indicating wild-type status and susceptibility to dapsone and rifampicin, respectively. A mutation was identified at codon 91 (alanine [GCA] \u2192 valine [GTA]) in the\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      gene (20%), resulting in an alanine-to-valine change known to cause resistance to ofloxacin. Five samples did not provide adequate chromatogram quality for analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      The study identified mutations in the\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      gene which is associated with ofloxacin resistance in\n                      <jats:italic toggle=\"yes\">M. leprae<\/jats:italic>\n                      in Central India. While the absence of resistance to first-line anti-leprosy drugs is reassuring, the emergence of resistance to fluoroquinolones is a cause for concern. Early detection of resistant strains facilitates prompt initiation of drug therapies, reducing their spread and advancing the global leprosy eradication effort.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_35_25","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T15:00:19Z","timestamp":1750431619000},"page":"117-125","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Detection of Gene Mutation Related to Drug Resistance in Mycobacterium leprae"],"prefix":"10.4103","volume":"14","author":[{"given":"Saumya","family":"Shukla","sequence":"first","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaina","family":"Gaikwad","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhandapani","family":"Sureshgopi","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Genomics Laboratory, ICMR-National Institute of Research in Tribal Health, Jabalpur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek Kumar","family":"Chouksey","sequence":"additional","affiliation":[{"name":"Department of Central Instrumentation Facility, ICMR-National Institute of Research in Tribal Health, Jabalpur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrashekhar","family":"Pathe","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerene","family":"Mathews","sequence":"additional","affiliation":[{"name":"Department of Dermatology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitendra","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashank","family":"Purwar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasis","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pushpendra","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Genomics Laboratory, ICMR-National Institute of Research in Tribal Health, Jabalpur, India"},{"name":"Model Rural Health Research Unit (MRHRU) Badoni, Datia, Madhya Pradesh, India"},{"name":"Faculty of Medical Research, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4827-919X","authenticated-orcid":false,"given":"Anand Kumar","family":"Maurya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institutes of Medical Sciences, Bhopal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,6,20]]},"reference":[{"key":"R2-20260422","doi-asserted-by":"crossref","first-page":"697","DOI":"10.4103\/idoj.idoj_245_22","article-title":"Recording of leprosy cases in India: Need for a user-friendly and effective app","volume":"13","author":"Bahunuthula","year":"2022","journal-title":"Indian Dermatol Online J"},{"key":"R3-20260422","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4103\/idoj.IDOJ_282_17","article-title":"Current situation of leprosy in India and its future implications","volume":"9","author":"Rao","year":"2018","journal-title":"Indian Dermatol Online J"},{"key":"R4-20260422","first-page":"255","article-title":"Classification of leprosy according to immunity. 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