{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T13:19:50Z","timestamp":1783430390889,"version":"3.54.6"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,4]]},"abstract":"\n Background:<\/jats:title>\n \n The\n Mycobacterium tuberculosis<\/jats:italic>\n complex (MTBC) includes human-and animal-adapted species. Routine TrueNat\u2122 testing detects MTBC but does not differentiate subspecies. This study evaluated the reuse of residual TrueNat\u2122 DNA eluates for MTBC subspecies identification.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n A cross-sectional laboratory feasibility study was performed using residual DNA (~78 \u00b5L) from 1,115 high-bacillary-load TrueNat\u2122 MTBC-positive clinical specimens collected between 2022 and 2025. A two-step real-time polymerase chain reaction (PCR) assay targeting IS1081, MTCHum, MTCAni, and species-specific loci for\n Mycobacterium bovis<\/jats:italic>\n (Mbov),\n Mycobacterium caprae<\/jats:italic>\n (Mcap), and\n Mycobacterium orygis<\/jats:italic>\n (Morg) was performed. All Mbov detections underwent confirmatory PCR and culture.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n All eluates produced valid amplification. Of 1,115 samples, 1,114 (99.91%) were identified as\n M. tuberculosis<\/jats:italic>\n sensu stricto and one (0.09%) as Mbov. The Mbov isolate, obtained from a 2-year-old child with lymphadenitis, was confirmed to be a Bacillus Calmette\u2013Gu\u00e9rin (BCG) vaccine strain rather than wild-type Mbov. No Mcap or Morg were detected. Negative controls remained negative, and repeat testing showed 100% concordance.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n Residual DNA from routine TrueNat\u2122 testing can be effectively reused for MTBC subspecies differentiation. This implementation feasibility study demonstrates that residual DNA from routine TrueNat\u2122 testing can be reused for MTBC subspecies differentiation within existing workflows without additional specimen collection or DNA extraction. Dedicated validation and epidemiologic studies are required to evaluate its utility in surveillance applications.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_44_26","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:18:00Z","timestamp":1782857880000},"page":"115-122","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Reuse of Residual DNA Eluates from TrueNat\u2122 Testing for Mycobacterium tuberculosis Complex Subspecies Differentiation: A Proof-of-concept Implementation Feasibility Study"],"prefix":"10.4103","volume":"15","author":[{"given":"Hindol","family":"Maity","sequence":"first","affiliation":[{"name":"Department of Microbiology and Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research (DMIHER), Sawangi (Meghe), Sevagram, Wardha, Maharashtra, India"},{"name":"Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research (DMIHER), Sawangi (Meghe), Sevagram, Wardha, Maharashtra, India"},{"name":"Department of Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keshao","family":"Hiwale","sequence":"additional","affiliation":[{"name":"Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research (DMIHER), Sawangi (Meghe), Sevagram, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Meshram","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Jawaharlal Nehru Medical College, Datta Meghe Institute of Higher Education and Research (DMIHER), Sawangi (Meghe), Sevagram, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohan","family":"Papanna","sequence":"additional","affiliation":[{"name":"Department of Huck Institute of Life Sciences, Pennsylvania State University, University Park, Pennsylvania, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijayshri","family":"Deotale","sequence":"additional","affiliation":[{"name":"Department of Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratibha","family":"Narang","sequence":"additional","affiliation":[{"name":"Department of Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences (MGIMS), Sevagram, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"R1-20260707","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-319-64371-7_1","article-title":"The nature and evolution of genomic diversity in the Mycobacterium tuberculosis complex","volume":"1019","author":"Brites","year":"2017","journal-title":"Adv Exp Med Biol"},{"key":"R2-20260707","doi-asserted-by":"crossref","first-page":"100495","DOI":"10.1016\/j.onehlt.2023.100495","article-title":"Bovine tuberculosis in India: The need for one health approach and the way forward","volume":"16","author":"Ramanujam","year":"2023","journal-title":"One Health"},{"key":"R3-20260707","doi-asserted-by":"crossref","first-page":"466","DOI":"10.4103\/ijmy.ijmy_148_22","article-title":"Complicated clinical course of zoonotic tuberculosis due to Mycobacterium caprae: A case report and literature 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implications","volume":"8","author":"Maity","year":"2026","journal-title":"Infect Dis Clin Microbiol"},{"key":"R7-20260707","doi-asserted-by":"crossref","first-page":"e0339024","DOI":"10.1371\/journal.pone.0339024","article-title":"Association between livestock exposures and human tuberculosis in Wardha, Central India: An exploratory case-control study","volume":"21","author":"Maity","year":"2026","journal-title":"PLoS One"},{"key":"R8-20260707","doi-asserted-by":"crossref","first-page":"390","DOI":"10.4103\/ijmy.ijmy_151_25","article-title":"Agreement between TrueNat Mycobacterium tuberculosis\/rifampicin and microscopy for detection of pulmonary and extrapulmonary tuberculosis at a tertiary care hospital of Eastern Uttar Pradesh, India","volume":"14","author":"Mohanty","year":"2025","journal-title":"Int J Mycobacteriol"},{"key":"R9-20260707","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ijmyco.2014.04.003","article-title":"Evaluation of the Indian TrueNAT micro 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