{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:59:46Z","timestamp":1793408386796,"version":"4.5.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Mycobacterial fatty acid synthase Type-II (FAS-II) components are major virulence factors exploited as potential targets for developing novel antimycobacterial drugs. The FAS-II enzyme 3-hydroxyacyl-ACP dehydratase (HadC) is important for biofilm development and pathogenesis of\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      and other mycobacterial species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Literature review and homology search led to the identification of\n                      <jats:italic toggle=\"yes\">Mycobacterium fortuitum<\/jats:italic>\n                      MF\n                      <jats:italic toggle=\"yes\">hadC<\/jats:italic>\n                      gene. Functional interaction study of MFHadC protein was done using STRING.\n                      <jats:italic toggle=\"yes\">M. fortuitum<\/jats:italic>\n                      MF\n                      <jats:italic toggle=\"yes\">hadC<\/jats:italic>\n                      over-expressing (HS) and knockdown (HA) strains were constructed and validated by expression analysis using quantitative polymerase chain reaction. The strains were analyzed for growth behavior and surface spreading ability. Biofilm formation was assayed through crystal violet assay, viability count, and basic fuchsin staining. In addition, survival of the strains was studied under\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      nutrient starvation and detergent stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>STRING analysis showed the interaction of HadC with proteins involved in biofilm formation. The strains HS and HA showed spreading ability on the agarose surface, exhibiting translocation patterns similar to the vector control strain. All three strains showed a similar amount of biofilm formation when analyzed using crystal violet assay, viability count, and basic fuchsin staining. The strains showed no deviation in survival when incubated under nutrient starvation and detergent stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      Our results suggest that MF\n                      <jats:italic toggle=\"yes\">hadC<\/jats:italic>\n                      may not be important for the formation and maintenance of biofilm, a factor critically important in\n                      <jats:italic toggle=\"yes\">M. fortuitum<\/jats:italic>\n                      pathogenicity. However, not essential for survival and growth, MF\n                      <jats:italic toggle=\"yes\">hadC<\/jats:italic>\n                      maintains the viability of\n                      <jats:italic toggle=\"yes\">M. fortuitum<\/jats:italic>\n                      under a nutrient-starved environment. Collectively, MF\n                      <jats:italic toggle=\"yes\">hadC<\/jats:italic>\n                      may not be used as a biofilm-specific marker for\n                      <jats:italic toggle=\"yes\">M. fortuitum<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_46_22","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T06:10:54Z","timestamp":1655187054000},"page":"159-166","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":13,"title":["Knockdown of the Type-II Fatty Acid Synthase Gene hadC in Mycobacterium fortuitum Does Not Affect Its Growth, Biofilm Formation, and Survival under 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