{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:05:14Z","timestamp":1790867114924,"version":"4.1.0"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4]]},"abstract":"\n Background:<\/jats:title>\n \n Recently, moxifloxacin (MFX)-resistant results of\n Mycobacterium tuberculosis<\/jats:italic>\n (\n Mtb<\/jats:italic>\n ) obtained by GenoType MTBDR\n sl<\/jats:italic>\n (second-line line probe assay [SL-LPA]) have been stratified to determine their resistance level; however, its accuracy has not been well studied. Therefore, the study aimed to evaluate the diagnostic accuracy of SL-LPA, with phenotypic drug susceptibility testing (pDST) and whole-genome sequencing (WGS) for the detection of MFX-resistant\n Mtb<\/jats:italic>\n and their resistance level.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n A total of 111 sputum samples were subjected to SL-LPA according to the diagnostic algorithm of the National Tuberculosis Elimination Program. Results were compared with pDST of MFX (at critical concentration [CC, 0.25 \u03bcg\/ml] and clinical breakpoint [CB, 1.0 \u03bcg\/ml] using BACTEC mycobacterial growth indicator tube-960), and WGS.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n At CC, SL-LPA and pDST yielded concordant results of MFX for 104 of 111 (94%). However, at CB, 23 of 30 (77%) isolates carrying\n gyrA<\/jats:italic>\n mutation known to confer low-level resistance to MFX were scored as susceptible by pDST. Among 46\n Mtb<\/jats:italic>\n isolates carrying\n gyrA<\/jats:italic>\n mutations known to confer high-level resistance to MFX, 36 (78%) isolates yielded concordant results, while 10 (22%) isolates were scored as susceptible at CB by pDST. WGS identified\n gyrA<\/jats:italic>\n mutations in all isolates suggested by SL-LPA.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n It is concluded that the stratification of MFX-resistant results by SL-LPA\/genotypic method is not very well correlated with pDST (at CB), and hence, pDST may not be completely replaced by SL-LPA.\n gyrA<\/jats:italic>\n D94G and\n gyrA<\/jats:italic>\n A90V are the most prevalent mutations in MFX-resistant\n Mtb.<\/jats:italic>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijmy.ijmy_70_22","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T06:10:54Z","timestamp":1655187054000},"page":"183-189","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":11,"title":["Diagnostic Utility of GenoType MTBDRsl Assay for the Detection of Moxifloxacin-Resistant Mycobacterium tuberculosis, as Compared to Phenotypic Method and Whole-Genome Sequencing"],"prefix":"10.4103","volume":"11","author":[{"given":"Raj Narayan","family":"Yadav","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manpreet","family":"Bhalla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavish","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grish C.","family":"Sah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra Kumar","family":"Dewan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Singhal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R2-8-20240902","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1128\/AAC.01049-15","article-title":"Mutations in gyrA 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new and previously treated pulmonary tuberculosis cases from Aligarh region of Northern India","volume":"7","author":"Ahmed","year":"2018","journal-title":"Int J Mycobacteriol"},{"key":"R16-8-20240902","doi-asserted-by":"crossref","first-page":"296","DOI":"10.4103\/ijmy.ijmy_61_17","article-title":"Rapid laboratory diagnosis of pulmonary tuberculosis","volume":"6","author":"Bhirud","year":"2017","journal-title":"Int J Mycobacteriol"},{"key":"R18-8-20240902","doi-asserted-by":"crossref","first-page":"66","DOI":"10.4103\/0974-777X.154443","article-title":"Utility of MPT64 antigen detection for rapid confirmation of Mycobacterium tuberculosis complex","volume":"7","author":"Arora","year":"2015","journal-title":"J Glob Infect Dis"},{"key":"R21-8-20240902","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1128\/JCM.03073-14","article-title":"Mycobacterial DNA extraction for whole-genome sequencing from early positive liquid (MGIT) 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