{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:22:47Z","timestamp":1787764967472,"version":"build-2784847793"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To report the histopathologic correlates of trabecular meshwork (TM) specimens procured by microincisional trabeculectomy (MIT) for different severities of glaucoma (early glaucoma: visual field mean deviation [MD] &lt;\u22126 dB, moderate glaucoma: MD from \u2212 6 to \u2212 12 dB, and advanced glaucoma: MD &lt;\u221212 dB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>TM specimens from four patients undergoing MIT with or without cataract surgery were analyzed by routine histopathology for structural changes. The number of cells, the number of cells with spindle-shaped nuclei suggestive of epithelial\u2013mesenchymal transformation (EMT), and the distance between the trabecular beams were calculated using different tools on freely available ImageJ software using the line or pint\/count tool.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The TM specimens procured from two early and two advanced glaucoma cases showed decreasing cellularity and decreased compact arrangement of the trabecular beams in severe disease stages. The number of cells and preserved architecture in all four specimens were evident, with &gt; 50 cells being present per section in all four cases despite the glaucoma being of advanced disease stage in two patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The TM specimens obtained from MIT can be utilized for downstream analysis using different molecular methods for studying the molecular events in the tissue from early to severe glaucoma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1390_23","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T05:01:04Z","timestamp":1702616464000},"page":"335-338","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":6,"title":["Histopathologic correlates of trabecular meshwork in microincisional trabeculectomy"],"prefix":"10.4103","volume":"72","author":[{"given":"Aparna","family":"Rao","sequence":"first","affiliation":[{"name":"Glaucoma Service, LV Prasad Eye Institute, MTC Campus, Patia, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"R1-20260504","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.exer.2008.11.025","article-title":"Structural changes of the trabecular meshwork in different kinds of glaucoma","volume":"88","author":"Tektas","year":"2009","journal-title":"Exp Eye Res"},{"key":"R2-20260504","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/0002-9394(60)91620-2","article-title":"Histopathology of the trabecular meshwork in glaucoma","volume":"49","author":"WOLTER","year":"1960","journal-title":"Am J Ophthalmol"},{"key":"R3-20260504","doi-asserted-by":"crossref","first-page":"e217","DOI":"10.1016\/j.jfo.2020.05.002","article-title":"The trabecular meshwork: Structure, function and clinical implications. A review of the literature","volume":"43","author":"Buffault","year":"2020","journal-title":"J Fr Ophtalmol"},{"key":"R4-20260504","doi-asserted-by":"crossref","first-page":"8849","DOI":"10.1167\/iovs.11-7591","article-title":"Histopathology of the trabecular meshwork and Schlemm\u2019s canal in primary angle-closure glaucoma","volume":"52","author":"Hamanaka","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"R5-20260504","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1097\/00061198-199704000-00009","article-title":"Severity of optic nerve damage in eyes with POAG is correlated with changes in the trabecular meshwork","volume":"6","author":"Gottanka","year":"1997","journal-title":"J Glaucoma"},{"key":"R6-20260504","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/S0161-6420(84)34248-8","article-title":"Trabecular meshwork cellularity in primary open-angle glaucoma and non-glaucomatous normals","volume":"91","author":"lvarado","year":"1984","journal-title":"Ophthalmology"},{"key":"R7-20260504","doi-asserted-by":"crossref","first-page":"3448.","DOI":"10.3390\/cells10123448","article-title":"Primary human trabecular meshwork model for pseudoexfoliation","volume":"10","author":"Chakraborty","year":"2021","journal-title":"Cells"},{"key":"R8-20260504","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.arr.2016.05.012","article-title":"From DNA damage to functional changes of the trabecular meshwork in aging and glaucoma","volume":"29","author":"Sacc\u00e0","year":"2016","journal-title":"Ageing Res Rev"},{"key":"R9-20260504","doi-asserted-by":"crossref","first-page":"e0286020.","DOI":"10.1371\/journal.pone.0286020","article-title":"\u2018Microincisional trabeculectomy for glaucoma\u201d","volume":"18","author":"Rao","year":"2023","journal-title":"PLoS One"},{"key":"R10-20260504","doi-asserted-by":"crossref","first-page":"138.","DOI":"10.1186\/s12886-021-01895-6","article-title":"Sampling trabecular meshwork using TrabEx","volume":"21","author":"Ramjiani","year":"2021","journal-title":"BMC Ophthalmol"},{"key":"R11-20260504","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.ajo.2018.05.028","article-title":"Histologic analysis of trabecular meshwork obtained from Kahook dual blade goniotomy","volume":"192","author":"Swaminathan","year":"2018","journal-title":"Am J Ophthalmol"},{"key":"R12-20260504","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1097\/01.ijg.0000196653.77836.af","article-title":"Ab interno trabeculectomy: Development of a novel device (Trabectome) and surgery for open-angle glaucoma","volume":"15","author":"Francis","year":"2006","journal-title":"J Glaucoma"}],"container-title":["Indian Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/IJO.IJO_1390_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:54:02Z","timestamp":1787763242000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/IJO.IJO_1390_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,15]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/ijo.ijo_1390_23","relation":{},"ISSN":["0301-4738","1998-3689"],"issn-type":[{"value":"0301-4738","type":"print"},{"value":"1998-3689","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,15]]},"assertion":[{"value":"2023-05-28","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-21","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}