{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:42:07Z","timestamp":1786653727744,"version":"build-2736575974"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To compare the histomorphologic changes on the anterior lens capsule by both epithelial and basement membrane side staining to those of only basement membrane side staining of the anterior lens capsule with Trypan Blue (TB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A cross-sectional study was done on 72 samples from patients who underwent cataract surgery between April 2021 and September 2022. After capsulorhexis of the TB-stained capsule, it was made into two halves externally and one half labeled as controls (sample A). The other half was immediately stained further with TB on the epithelial side and was taken as cases (sample B). Samples were analyzed for lens epithelial cells and basement membrane changes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The loss of intactness of lens epithelial cells, partial or complete detachment of lens epithelial cells, degeneration of lens epithelial cells, and basement edema were significantly higher in cases compared to controls, whereas intactness of the basement membrane did not show any statistical significance between the two groups. There was a statistically significant decrease in cell density in cases compared to controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Staining the epithelial side of the capsular bag with TB is more detrimental to lens epithelial cells and paves the way for a further study of staining the capsular bag before intra-ocular lens implantation to reduce the incidence of posterior capsule opacification.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1558_23","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T10:00:39Z","timestamp":1703584839000},"page":"578-581","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Comparative histomorphologic study of basement membrane side staining and additional epithelial side staining of the anterior lens capsule with Trypan Blue"],"prefix":"10.4103","volume":"72","author":[{"given":"Kommana","family":"Srinija","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, K. S. Hegde Medical Academy of Nitte DU, Deralakatte, Mangalore, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subrahmanya K","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, K. S. Hegde Medical Academy of Nitte DU, Deralakatte, Mangalore, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kishan H L","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Pathology, K. S. Hegde Medical Academy of Nitte DU, Deralakatte, Mangalore, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"R1-20260504","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1016\/S0886-3350(03)00225-6","article-title":"Pharmacologic prevention of posterior capsule opacification: In vitro effects of preservative-free lidocaine 1% on lens epithelial cells","volume":"29","author":"Vargas","year":"2003","journal-title":"J Cataract Refract Surg"},{"key":"R2-20260504","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.4103\/ijo.IJO_454_17","article-title":"Long-term results of trypan blue dye irrigation in the capsular bag to prevent posterior capsule opacification: A randomized trial","volume":"65","author":"Joshi","year":"2017","journal-title":"Indian J 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Health"},{"key":"R12-20260504","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.jcrs.2009.11.005","article-title":"Trypan blue staining for capsulorhexis: Ultrastructural effect on lens epithelial cells and capsules","volume":"36","author":"Portes","year":"2010","journal-title":"J Cataract Refract Surg"},{"key":"R13-20260504","article-title":"Get the best results with trypan blue dye staining of anterior lens capsule: Laser spectroscopy study","author":"Mathen","year":"2015"},{"key":"R14-20260504","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/j.jcrs.2008.03.041","article-title":"Effect of trypan blue on the elasticity of the human anterior lens capsule","volume":"34","author":"Dick","year":"2008","journal-title":"J Cataract Refract Surg"},{"key":"R15-20260504","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.curtheres.2006.12.003","article-title":"Effects of trypan blue on corneal endothelium and anterior lens capsule in albino Wistar rats: An investigator-masked, controlled, two-period, experimental study","volume":"67","author":"Ari","year":"2006","journal-title":"Curr Ther Res Clin Exp"},{"key":"R16-20260504","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1136\/bjo.2004.044537","article-title":"Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells","volume":"88","author":"Kwok","year":"2004","journal-title":"Br J Ophthalmol"},{"key":"R17-20260504","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1001\/archopht.122.5.736","article-title":"Biocompatibility of trypan blue with human corneal cells","volume":"122","author":"Van Dooren","year":"2004","journal-title":"Arch Ophthalmol"}],"container-title":["Indian Journal of 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