{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:21:24Z","timestamp":1787761284770,"version":"build-2784847793"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,8]]},"abstract":"\n Purpose:<\/jats:title>\n To compare the outcomes of two techniques, for preparation of microkeratome-assisted ultrathin grafts for Descemet's stripping automated endothelial keratoplasty (DSAEK).<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n The study involved 20 eyes of 20 patients with pseudophakic bullous keratopathy, randomized into two groups. Group 1 eyes underwent microkeratome-assisted DSAEK using the single-pass technique for lenticule preparation, whereas group 2 eyes underwent microkeratome-assisted DSAEK using the double-pass technique. Patients were followed up till 6 months, postoperatively. Best-corrected visual acuity (BCVA) at final follow-up was considered as the primary outcome measure, whereas graft thickness (GT) contrast sensitivity and endothelial cell loss were considered as the secondary outcome measures. A\n P<\/jats:italic>\n value of <0.05 was considered as statistically significant.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Baseline characteristics of two groups were comparable. The mean central GT was comparable in both groups at 6 months follow-up [group 1: 98 \u00b1 24.46 \u03bcm, group 2: 129 \u00b1 31.46 \u03bcm (\n P<\/jats:italic>\n = 0.18)]. Both groups fared equally in terms of BCVA (\n P<\/jats:italic>\n = 0.33). Contrast sensitivity was significantly better in group 1 eyes (\n P<\/jats:italic>\n = 0.045). A statistically significant negative correlation was found between postoperative BCVA and postoperative GT (R = \u22120.728,\n P<\/jats:italic>\n = 0.016). The percentage endothelial cell loss was slightly higher in group 2 eyes, although not statistically significant. Two eyes in group 2 experienced complications during lenticule preparation. None of the eye experienced any complication in the postoperative period.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Both techniques provided grafts with comparable thickness and endothelial cell loss and were associated with comparable BCVA, at final follow-up visit. The contrast sensitivity was, however, better in eyes receiving grafts prepared with the single-pass technique.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1660_18","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T06:42:21Z","timestamp":1564123341000},"page":"1289-1294","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Microkeratome-assisted ultrathin Descemet's stripping automated endothelial keratoplasty: A randomized trial comparing single-pass versus double-pass technique"],"prefix":"10.4103","volume":"67","author":[{"given":"Namrata","family":"Sharma","sequence":"first","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aarifa Yasmin","family":"Hussain","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Nagpal","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anubha","family":"Rathi","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prafulla K","family":"Maharana","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Sinha","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tushar","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeewan Singh","family":"Titiyal","sequence":"additional","affiliation":[{"name":"Cornea, Cataract and Refractive Surgery Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20251013","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1016\/j.ophtha.2009.06.021","article-title":"Descemet's stripping endothelial keratoplasty: safety and outcomes: A report by the American Academy of Ophthalmology","volume":"116","author":"Lee","year":"2009","journal-title":"Ophthalmology"},{"key":"R2-20251013","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1016\/j.ophtha.2009.07.010","article-title":"Descemet's membrane endothelial keratoplasty: Prospective multicenter study of visual and refractive outcomes and endothelial survival","volume":"116","author":"Price","year":"2009","journal-title":"Ophthalmology"},{"key":"R3-20251013","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.ophtha.2011.09.019","article-title":"Risk of corneal transplant rejection significantly reduced with Descemet's membrane endothelial keratoplasty","volume":"119","author":"Anshu","year":"2012","journal-title":"Ophthalmology"},{"key":"R4-20251013","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1016\/j.ophtha.2011.06.002","article-title":"Descemet's membrane endothelial keratoplasty: Prospective study of 1-year visual outcomes, graft survival, and endothelial cell loss","volume":"118","author":"Guerra","year":"2011","journal-title":"Ophthalmology"},{"key":"R5-20251013","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1097\/ICO.0b013e3181f236c6","article-title":"Comparison of central corneal graft thickness to visual acuity outcomes in endothelial keratoplasty","volume":"30","author":"Neff","year":"2011","journal-title":"Cornea"},{"key":"R6-20251013","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1167\/iovs.11-7753","article-title":"Microkeratome assisted preparation of ultrathin grafts for descemet stripping automated endothelial keratoplasty","volume":"53","author":"Busin","year":"2012","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"R7-20251013","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.ophtha.2012.11.030","article-title":"Ultrathin Descemet's stripping automated endothelial keratoplasty with the microkeratome double-pass technique. Two-year outcomes","volume":"120","author":"Busin","year":"2013","journal-title":"Ophthalmology"},{"key":"R8-20251013","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1097\/ICO.0000000000000603","article-title":"Postoperative graft thickness obtained with single-pass microkeratome-assisted ultrathin Descemet stripping automated endothelial keratoplasty","volume":"34","author":"Nahum","year":"2015","journal-title":"Cornea"},{"key":"R9-20251013","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/ceo.12271","article-title":"Thin lenticule Descemet's stripping automated endothelial keratoplasty: Single, slow pass technique","volume":"42","author":"Vajpayee","year":"2014","journal-title":"Clin Experiment Ophthalmol"},{"key":"R10-20251013","first-page":"425","article-title":"Double-pass microkeratome technique for ultra-thin graft preparation in Descemet's stripping automated endothelial keratoplasty","volume":"6","author":"Hsu","year":"2012","journal-title":"Clin Ophthalmol"},{"key":"R11-20251013","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1097\/ICL.0000000000000243","article-title":"Comparative evaluation of use of 400-\u03bcm and 350-\u03bcm microkeratome head to prepare donor tissue in cases of Descemet stripping automated endothelial keratoplasty","volume":"43","author":"Jain","year":"2017","journal-title":"Eye Contact Lens"},{"key":"R12-20251013","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s00347-013-2795-0","article-title":"Influence of donor lamella thickness on visual acuity after Descemet's stripping automated endothelial keratoplasty (DSAEK)","volume":"111","author":"Maier","year":"2014","journal-title":"Ophthalmologe"}],"container-title":["Indian Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/02223307-201967080-00008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:30:39Z","timestamp":1787758239000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/02223307-201967080-00008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8]]},"references-count":12,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.4103\/ijo.ijo_1660_18","relation":{},"ISSN":["0301-4738","1998-3689"],"issn-type":[{"value":"0301-4738","type":"print"},{"value":"1998-3689","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8]]},"assertion":[{"value":"2018-10-03","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-06","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}