{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:32:33Z","timestamp":1786649553557,"version":"build-2736575974"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 3","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,6]]},"abstract":"\n Purpose:<\/jats:title>\n \n To review 27 cases of microbial keratitis caused by\n Lasiodiplodia theobromae<\/jats:italic>\n presenting to a tertiary eye care center in eastern India over seven-year period and analyze the risk factors, clinical profile, microbiological characteristics, treatment, and outcome of patients.\n <\/jats:p>\n <\/jats:sec>\n \n Method:<\/jats:title>\n \n This is a retrospective review of 27 cases of microbial keratitis diagnosed with\n Lasiodiplodia theobromae<\/jats:italic>\n between November 2015 and October 2022. Data were retrieved using the electronic medical record system. Additional outcomes such as average time of resolution, frequency, type, and outcome of surgical interventions, wherever performed, were also analyzed. Descriptive statistics were used to interpret the demographic data.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The mean age of patients was 56.8 \u00b1 18.7 years (range: 23\u201386), the male: female ratio was 2.4:1, and the average follow-up was 90.2 days. Eleven (40.7%) patients had a history of trauma, and 16 (59.3%) presented with a large infiltrate (>6 mm). Samples from all the patients were culture-positive for\n Lasiodiplodia theobromae<\/jats:italic>\n , of which 26 (96.3%) were corneal scrapings and one (4%) was a half-corneal button. Eight (29.6%) patients had coexisting bacterial infections. Successful management was achieved in 12 eyes (66.7%) with medical treatment alone (topical natamycin,\n n<\/jats:italic>\n = 7; topical natamycin + oral ketoconazole,\n n<\/jats:italic>\n = 3; and topical natamycin + topical gatifloxacin,\n n<\/jats:italic>\n = 2). However, surgical intervention was required for nine (33.3%) eyes.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Medical management with natamycin was effective in treating patients with\n Lasiodiplodia<\/jats:italic>\n keratitis. However, advanced cases necessitated surgical intervention for complete resolution.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1677_24","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T07:00:25Z","timestamp":1744873225000},"page":"S394-S398","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Lasiodiplodia keratitis: A case series of 27 patients"],"prefix":"10.4103","volume":"73","author":[{"given":"Smruti R","family":"Priyadarshini","sequence":"first","affiliation":[{"name":"Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alluri","family":"Himabindu","sequence":"additional","affiliation":[{"name":"Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srikant K","family":"Sahu","sequence":"additional","affiliation":[{"name":"Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrita","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himanshu S","family":"Behera","sequence":"additional","affiliation":[{"name":"Microbiology Services, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujata","family":"Das","sequence":"additional","affiliation":[{"name":"Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"R1-20260422","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jtos.2021.12.001","article-title":"Fungal keratitis: A review of clinical presentations, treatment strategies and outcomes","volume":"24","author":"Sharma","year":"2022","journal-title":"Ocul Surf"},{"key":"R2-20260422","first-page":"214","article-title":"Corneal blindness: A global perspective","volume":"79","author":"Whitcher","year":"2001","journal-title":"Bull World Health Organ"},{"key":"R3-20260422","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1136\/bjophthalmol-2014-305000","article-title":"Trends in bacterial and fungal keratitis in South India, 2002\u20132012","volume":"99","author":"Lalitha","year":"2015","journal-title":"Br J Ophthalmol"},{"key":"R4-20260422","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s12281-013-0150-1","article-title":"Current thoughts in fungal keratitis: Diagnosis and treatment","volume":"7","author":"Ansari","year":"2013","journal-title":"Curr Fungal Infect Rep"},{"key":"R5-20260422","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1128\/CMR.16.4.730-797.2003","article-title":"Current perspectives on ophthalmic mycoses","volume":"16","author":"Thomas","year":"2003","journal-title":"Clin Microbiol Rev"},{"key":"R6-20260422","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1097\/00003226-200210000-00002","article-title":"Evaluation of corneal scraping smear examination methods in the diagnosis of bacterial and fungal keratitis: A survey of eight years of laboratory experience","volume":"21","author":"Sharma","year":"2002","journal-title":"Cornea"},{"key":"R7-20260422","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1136\/bjo.79.11.1024","article-title":"Causes of suppurative keratitis in Ghana","volume":"79","author":"Hagan","year":"1995","journal-title":"Br J Ophthalmol"},{"key":"R8-20260422","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1136\/bjo.81.11.965","article-title":"Epidemiology and aetiological diagnosis of corneal ulceration in Madurai, south India","volume":"81","author":"Srinivasan","year":"1997","journal-title":"Br J Ophthalmol"},{"key":"R9-20260422","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1097\/00003226-200208000-00004","article-title":"The epidemiological features and laboratory results of fungal keratitis: A 10-year review at a referral eye care center in South India","volume":"21","author":"Gopinathan","year":"2002","journal-title":"Cornea"},{"key":"R10-20260422","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.mmcr.2015.03.002","article-title":"An unusual skin lesion caused by Lasiodiplodia theobromae","volume":"8","author":"Papacostas","year":"2015","journal-title":"Med Mycol Case Rep"},{"key":"R11-20260422","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.funbio.2016.07.006","article-title":"Analysis of phylogeny, distribution, and pathogenicity of botryosphaeriaceae species associated with gummosis of anacardium in Brazil, with a new species of Lasiodiplodia","volume":"121","author":"Netto","year":"2017","journal-title":"Fungal Biol"},{"key":"R12-20260422","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1094\/PDIS-03-13-0245-RE","article-title":"Population genetic analysis reveals diversity in lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE","volume":"97","author":"Al-Sadi","year":"2013","journal-title":"Plant Dis"},{"key":"R13-20260422","first-page":"11","article-title":"Mycotic infections of the cornea","volume":"15","author":"Puttanna","year":"1967","journal-title":"J All India Ophthalmol Soc"},{"key":"R14-20260422","doi-asserted-by":"crossref","first-page":"457","DOI":"10.4103\/0255-0857.195369","article-title":"Banana peel culture as an indigenous medium for easy identification of late-sporulation human fungal pathogens","volume":"34","author":"Kindo","year":"2016","journal-title":"Indian J Med Microbiol"},{"key":"R15-20260422","doi-asserted-by":"crossref","first-page":"e541.","DOI":"10.1097\/MD.0000000000000541","article-title":"Treatment with intrastromal and intracameral voriconazole in 2 eyes with Lasiodiplodia theobromae keratitis: Case reports","volume":"94","author":"Lekhanont","year":"2015","journal-title":"Medicine (Baltimore)"},{"key":"R16-20260422","doi-asserted-by":"crossref","first-page":"e000055","DOI":"10.1099\/acmi.0.000055","article-title":"First report of lasiodiplodia pseudotheobromae keratitis susceptible to voriconazole in an Indian mango grower","volume":"1","author":"Vanam","year":"2019","journal-title":"Access Microbiol"},{"key":"R17-20260422","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s11046-012-9546-7","article-title":"Lasiodiplodia theobromae keratitis: A case report and review of literature","volume":"174","author":"Saha","year":"2012","journal-title":"Mycopathologia"},{"key":"R18-20260422","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1007\/s11046-017-0234-5","article-title":"Antifungal susceptibility, morphological and molecular characterization of lasiodiplodia theobromae isolated from a patient with keratitis","volume":"183","author":"da Rosa","year":"2018","journal-title":"Mycopathologia"},{"key":"R19-20260422","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/BF00941769","article-title":"Endophthalmitis after Lasiodiplodia theobromae corneal abscess","volume":"235","author":"Borderie","year":"1997","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"R20-20260422","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/00362177685190231","article-title":"Lasiodiplodia theobromae as a cause of keratomycoses","volume":"14","author":"Rebell","year":"1976","journal-title":"Sabouraudia"}],"container-title":["Indian Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/IJO.IJO_1677_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:55:20Z","timestamp":1786647320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/IJO.IJO_1677_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,17]]},"references-count":20,"journal-issue":{"issue":"Suppl 3","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/ijo.ijo_1677_24","relation":{},"ISSN":["0301-4738","1998-3689"],"issn-type":[{"value":"0301-4738","type":"print"},{"value":"1998-3689","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,17]]},"assertion":[{"value":"2024-07-14","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}