{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:27:36Z","timestamp":1787758056012,"version":"build-2784847793"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,7]]},"abstract":"\n Purpose:<\/jats:title>\n Stickler syndrome is associated with the development of rhegmatogenous retinal detachment (RRD), and often presents with ocular, auditory, skeletal, and orofacial abnormalities. Molecular analysis has proven effective in diagnosis, confirmation and classification of the disease. We aimed to describe the utility of next-generation sequencing (NGS) in genetic analysis of four Indian families with suspected Stickler syndrome.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n The index cases presented with retinal detachment with family history. Genetic analysis in the index case was performed by next-generation sequencing of inherited retinal degeneration genes, and validated by Sanger sequencing followed by co-segregation analysis in the other family members.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Twenty patients were included for the genetic analysis (15 males and 5 females from four families). Clinical details were available for 15 patients (30 eyes). Fourteen eyes (11 patients) developed RRD. In the 16 eyes without RRD, 8 underwent barrage laser to lattice degeneration and 8 were under observation. Disease segregating heterozygous mutations with pathogenic\/likely pathogenic effect was identified in\n COL2A1<\/jats:italic>\n (c.4318-1G>A, c.141G>A, c.1221+1G>A for 3 families) and\n COL11A1<\/jats:italic>\n (c.1737+1 G>A for 1 family) gene. In addition to the mutation in the\n COL2A1<\/jats:italic>\n gene, a pathogenic heterozygous variant associated with risk for arrhythmogenic right ventricular cardiomyopathy (ARVC) was identified in one member.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n NGS testing confirmed the presence of the causative gene for Stickler syndrome in the index case followed by evaluation of family members and confirmation of genetic and ocular findings. We believe that this may be the first such report of families with RRD from India.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1833_21","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T06:28:32Z","timestamp":1657607312000},"page":"2578-2583","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Genetic testing in four Indian families with suspected Stickler syndrome"],"prefix":"10.4103","volume":"70","author":[{"given":"Suganya","family":"Kandeeban","sequence":"first","affiliation":[{"name":"SNONGC Department of Genetics and Molecular Biology, Vision Research Foundation, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaustubh","family":"Kandale","sequence":"additional","affiliation":[{"name":"Shri Bhagwan Mahavir Department of VitroRetinal Services, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porkodi","family":"Periyasamy","sequence":"additional","affiliation":[{"name":"SNONGC Department of Genetics and Molecular Biology, Vision Research Foundation, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna","family":"Bhende","sequence":"additional","affiliation":[{"name":"Shri Bhagwan Mahavir Department of VitroRetinal Services, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramod","family":"Bhende","sequence":"additional","affiliation":[{"name":"Shri Bhagwan Mahavir Department of VitroRetinal Services, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinnakaruppan","family":"Mathavan","sequence":"additional","affiliation":[{"name":"SNONGC Department of Genetics and Molecular Biology, Vision Research Foundation, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sripriya","family":"Sarangapani","sequence":"additional","affiliation":[{"name":"Shri Bhagwan Mahavir Department of VitroRetinal Services, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"B1-20240828","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1136\/jmg.36.5.353","article-title":"Clinical and molecular genetics of Stickler syndrome","volume":"36","author":"Snead","year":"1999","journal-title":"J Med Genet"},{"key":"B2-20240828","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1097\/00125817-200105000-00008","article-title":"Clinical features of hereditary progressive arthro-ophthalmopathy (Stickler syndrome):A survey","volume":"3","author":"Stickler","year":"2001","journal-title":"Genet 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