{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:36:32Z","timestamp":1786649792669,"version":"build-2736575974"},"reference-count":16,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>Here we describe a new, non-human, ex-vivo model (goat eye model) for training surgeons in DMEK surgeons. In a wet lab setting, goat eyes were used to obtain a pseudo-DMEK graft of 8 mm from the goat lens capsule that was injected into another goat eye with the same maneuvers described for human DMEK. The DMEK pseudo-graft can be easily prepared, stained, loaded, injected, and unfolded into the goat eye model reproducing the similar maneuvers used for DMEK in a human eye, except for the descemetorhexis, which cannot be performed. The pseudo-DMEK graft behaves similar to human DMEK graft and useful for surgeons to experience and understand steps of DMEK early in learning curve. The concept of a non-human ex-vivo eye model is simple and reproducible and obviates the need for human tissue and the issues of poor visibility in stored corneal tissue.<\/jats:p>","DOI":"10.4103\/ijo.ijo_1834_22","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T03:36:18Z","timestamp":1684294578000},"page":"2230-2233","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["A goat eye, wet lab model for training in Descemet membrane endothelial keratoplasty"],"prefix":"10.4103","volume":"71","author":[{"given":"Nidhi","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Cornea and Refractive Services, and Stem Cell Lab, Dr. Shroff\u2019s Charity Eye Hospital, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maninder","family":"Bhogal","sequence":"additional","affiliation":[{"name":"Cornea Unit, Guy\u2019s and St. Thomas\u2019 Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravin K","family":"Vaddavalli","sequence":"additional","affiliation":[{"name":"The Cornea Institute, L V Prasad Eye Institute, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Boldini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Semeraro","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhil","family":"Varshney","sequence":"additional","affiliation":[{"name":"Department of Cornea and Refractive Services, and Stem Cell Lab, Dr. Shroff\u2019s Charity Eye Hospital, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito","family":"Romano","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy"},{"name":"Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"R1-20251006","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1016\/j.ophtha.2011.06.002","article-title":"Descemet's membrane endothelial keratoplasty:prospective study of 1-year visual outcomes, graft survival, and endothelial cell loss","volume":"118","author":"Guerra","year":"2011","journal-title":"Ophthalmology"},{"key":"R2-20251006","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1097\/ICO.0000000000000822","article-title":"Visual outcomes after descemet membrane endothelial keratoplasty versus descemet stripping automated endothelial keratoplasty-comparison of specific matched pairs","volume":"35","author":"Droutsas","year":"2016","journal-title":"Cornea"},{"key":"R3-20251006","first-page":"CD012097","article-title":"Descemet's membrane endothelial keratoplasty (DMEK) versus Descemet's stripping automated endothelial keratoplasty (DSAEK) for corneal endothelial failure","volume":"6","author":"Stuart","year":"2018","journal-title":"Cochrane Database Syst Rev"},{"key":"R4-20251006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/ICO.0000000000001066","article-title":"Impact of surgical learning curve in descemet membrane endothelial keratoplasty on visual acuity gain","volume":"36","author":"Debellemani\u00e8re","year":"2017","journal-title":"Cornea"},{"key":"R5-20251006","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1097\/ICO.0000000000001553","article-title":"Descemet membrane endothelial keratoplasty learning curve for graft preparation in an eye bank using 645 donor corneas","volume":"37","author":"Parekh","year":"2018","journal-title":"Cornea"},{"key":"R6-20251006","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1016\/j.ophtha.2011.03.037","article-title":"Learning curve in descemet's membrane endothelial keratoplasty:First series of 135 consecutive cases","volume":"118","author":"Dapena","year":"2011","journal-title":"Ophthalmology"},{"key":"R7-20251006","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1136\/bjophthalmol-2015-308330","article-title":"Spring-action apparatus for fixation of eyeball (SAFE):A novel, cost-effective yet simple device for ophthalmic wet-lab training","volume":"100","author":"Ramakrishnan","year":"2016","journal-title":"Br J Ophthalmol"},{"key":"R8-20251006","first-page":"987","article-title":"Descemet membrane endothelial keratoplasty (DMEK)","volume":"25","author":"Melles","year":"2006","journal-title":"Cornea"},{"key":"R9-20251006","doi-asserted-by":"crossref","first-page":"108326","DOI":"10.1016\/j.exer.2020.108326","article-title":"The human Descemet's membrane and lens capsule:Protein composition and biomechanical properties","volume":"201","author":"Halfter","year":"2020","journal-title":"Exp Eye Res"},{"key":"R10-20251006","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1016\/S0886-3350(02)01327-5","article-title":"Formalininduced cataract in goat eyes as a surgical training model for phacoemulsification","volume":"28","author":"Sudan","year":"2002","journal-title":"J Cataract Refract Surg"},{"key":"R11-20251006","doi-asserted-by":"crossref","first-page":"e362","DOI":"10.1111\/aos.12371","article-title":"Asimple ex vivo model for teaching Descemet membrane endothelial keratoplasty","volume":"92","author":"Droutsas","year":"2014","journal-title":"Acta Ophthalmol"},{"key":"R12-20251006","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1097\/ICO.0000000000001604","article-title":"Simulation model for DMEK donor preparation","volume":"37","author":"Mittal","year":"2018","journal-title":"Cornea"},{"key":"R13-20251006","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1097\/ICO.0000000000001112","article-title":"Human ex vivo artificial anterior chamber model for practice DMEK surgery","volume":"36","author":"Vasquez","year":"2017","journal-title":"Cornea"},{"key":"R14-20251006","doi-asserted-by":"crossref","first-page":"e17","DOI":"10.1097\/ICO.0000000000001446","article-title":"Artificial anterior chamber model using human donor corneas for DMEK surgical training","volume":"37","author":"Fogla","year":"2018","journal-title":"Cornea"},{"key":"R15-20251006","doi-asserted-by":"crossref","first-page":"e179","DOI":"10.1111\/aos.13852","article-title":"Artificial chamber and 3D printed iris:A new wet lab model for teaching Descemet's membrane endothelial keratoplasty","volume":"97","author":"Famery","year":"2019","journal-title":"Acta Ophthalmol"},{"key":"R16-20251006","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1097\/ICO.0000000000001435","article-title":"Partitioning an artificial anterior chamber with a latex diaphragm to simulate anterior and posterior segment pressure dynamics:The 'DMEK Practice Stage,'Where surgeons can rehearse the 'DMEK Dance'.","volume":"37","author":"S\u00e1les","year":"2018","journal-title":"Cornea"}],"container-title":["Indian Journal of 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