{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:29:59Z","timestamp":1786728599267,"version":"build-2736575974"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>Age-related cataract (ARC) is one of the leading causes of blindness and visual impairment globally. The aim of this study was to explore the role of Notch 3 in ARC as well as the mechanisms underlying Notch 3 in ARC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      An\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      model was established to mimick the microenvironment of ARC, the lens epithelial cell line (LEC) B3 were treated with hydrogen peroxide (H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      ) once they reached approximately 80% confluence. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the proliferation of LEC B3. The mRNA of Notch 3 was detected via RNA extraction and quantitative real-time PCR (RT-qPCR). The protein level of Notch 3 was measured by western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The mRNA and protein expression of Notch 3 were evidently down-regulated in H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      -inducted LEC B3. Overexpression of Notch 3 increased the proliferation and suppressed the apoptosis of H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      -inducted LECs B3. The elevated level of Notch 3 decreased the reactive oxygen species (ROS) fluorescence intensity and decreased Fe\n                      <jats:sup>2+<\/jats:sup>\n                      and total Fe levels. In addition, Notch 3 up-regulation increased the levels of ferroptosis-related proteins GPX4 and SLC7A11. Notch 3 overexpression decreased the protein levels of p-PI3K, and p-AKT in H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      -treated LECs B3. PI3K\/AKT pathway inhibitor reversed the role effect of Notch 3 overexpression on the proliferation, apoptosis, and ferroptosis of LECs cells in ARC model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The present study identified that Notch 3 regulates ferroptosis of LECs B3 in ARC by PI3K\/AKT pathway, which might offer a novel biomarker for the future investigation of the target of ARC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_1918_25","type":"journal-article","created":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T13:00:30Z","timestamp":1769346030000},"page":"203-211","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Notch 3 regulates ferroptosis of lens epithelial cells in age-related cataract by PI3K\/AKT pathway"],"prefix":"10.4103","volume":"74","author":[{"given":"Jiasheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Wuhan NO.1 Hospital, Wuhan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Li Yuan Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Li Yuan Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, 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