{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:30:20Z","timestamp":1786649420180,"version":"build-2736575974"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>The main factor that causes cataracts is the increased oxidative stress and imbalance of an antioxidant defense mechanism, which leads to significant changes in the lens microarchitecture. Senile cataract is the most common type of acquired cataracts due to aging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We carried out a case\u2013control study in the biochemistry department to examine the antioxidant status (catalase and total antioxidant capacity [TAC]) and lipid peroxidation marker, that is, malondialdehyde (MDA) in human lens epithelial cells (HLECs) of different grades of senile cortical, nuclear, and posterior subcapsular cataracts. We collected 150 samples from patients aged 50\u201390 years. These included 50 samples of cortical cataracts, 50 of nuclear cataracts, and 50 samples of posterior subcapsular cataracts. We measured catalase activity by the Beer method, TAC by the Benzie and Strain method, and protein by the Bradford method. We also estimated TAC in the aqueous extract of HLECs by the ferric reducing ability of plasma (FRAP) method and MDA by the thiobarbituric acid assay method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The results of this study showed that the level of catalase enzyme was higher in the first grade of nuclear, posterior subcapsular, and cortical cataracts than in other grades. This suggests that the catalase enzyme activity drops sharply in the second and third grades of these types of cataracts. The same pattern was observed for TAC, which was higher in the first grade of nuclear, posterior subcapsular, and cortical cataracts than in other grades. There were significant differences between catalase and TAC in different grades of cataracts, indicating that as the grading increases, both catalase and TAC decrease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The results of this study showed that the levels of MDA were higher and the levels of catalase and TAC were lower in patients with more severe cataracts compared to the healthy controls.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_19_24","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T11:00:54Z","timestamp":1727434854000},"page":"1506-1511","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of antioxidant status of lens epithelial cells in cataract patients"],"prefix":"10.4103","volume":"72","author":[{"given":"Kranti Santosh","family":"Sorte Gawali","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Seth GSMC and KEMH Parel Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash Namdeo","family":"Jadhao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Seth GSMC and KEMH Parel Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trupti Diwan","family":"Ramteke","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, G.M.C. Nandurbar, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam Jayant","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, T.N.M.C. and B.Y.L. Nair Hospital and Charitable Trust Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harshal","family":"Sahare","sequence":"additional","affiliation":[{"name":"Departments of Vitreo-Retina and Ocular Oncology and Cataract, and Phacorefractive Surgery, Sankara Eye Hospital, Shimoga, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"R2-20260427","first-page":"200","article-title":"Major changes in human ocular UV protection with age","volume":"42","author":"Bova","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"R3-20260427","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0014-4835(72)90057-7","article-title":"The behavior of reduced proteins from normal and cataractous lenses in highly dissociating media: Cross-linked protein in cataractous 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