{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:36:46Z","timestamp":1790613406847,"version":"4.1.0"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>\n                    Ocular microbiology deals with miniscule samples from ocular infections, which are difficult to collect, process, and analyze, necessitating special skills, and the knowledge of troubleshooting errors to reach a specific diagnosis. In this article, we highlight several practical points in ocular microbiology, common mistakes, and various approaches to resolve them. We have covered sample collection from different ocular compartments, processing for smear preparation and culture, transport of samples, staining and reagents issues, artifacts and contaminants, and interpretation of\n                    <jats:italic toggle=\"yes\">in-vitro<\/jats:italic>\n                    antimicrobial susceptibility testing reports. This review aims to help both ophthalmologists and microbiologists in making the practice of ocular microbiology and the interpretation of reports more reliable, hassle-free, and precise.\n                  <\/jats:p>","DOI":"10.4103\/ijo.ijo_2190_22","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T03:10:39Z","timestamp":1684293039000},"page":"1698-1705","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":10,"title":["Practical tips and common mistakes in ocular microbiology sampling and processing"],"prefix":"10.4103","volume":"71","author":[{"given":"Sanchita","family":"Mitra","sequence":"first","affiliation":[{"name":"Consultant Microbiologist, Jhaveri Microbiology Centre, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavya","family":"Chandran","sequence":"additional","affiliation":[{"name":"Consultant Ophthalmologist, Shantilal Shanghvi Cornea Institute, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merle","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Consultant Ophthalmologist, Shantilal Shanghvi Cornea Institute, LV Prasad Eye Institute, Kallam Anji Reddy Campus, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"R1-20251006","doi-asserted-by":"crossref","first-page":"67","DOI":"10.18773\/austprescr.2018.016","article-title":"Common eye infections","volume":"41","author":"Watson","year":"2018","journal-title":"Aust Prescr"},{"key":"R2-20251006","doi-asserted-by":"crossref","first-page":"e0007019","DOI":"10.1128\/CMR.00070-19","article-title":"Practical guidance for clinical microbiology laboratories:Diagnosis of ocular infections","volume":"34","author":"Leal","year":"2021","journal-title":"Clin Microbiol Rev"},{"key":"R3-20251006","doi-asserted-by":"crossref","first-page":"e000363","DOI":"10.1136\/bmjophth-2019-000363","article-title":"Does the sampling instrument influence corneal culture outcome in patients with infectious keratitis?A retrospective study comparing cotton tipped applicator with knife blade","volume":"5","author":"Sagerfors","year":"2020","journal-title":"BMJ Open Ophthalmol"},{"key":"R6-20251006","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/jcm7040073","article-title":"The effect of topical ocular anesthetic Proparacaine on conjunctival and nasal mucosal flora in dry eye disease patients","volume":"7","author":"Onerci Celebi","year":"2018","journal-title":"J Clin Med"},{"key":"R7-20251006","first-page":"21","article-title":"Corneal ulcer:Diagnosis and management","volume":"12","author":"Garg","year":"1999","journal-title":"Community Eye Health"},{"key":"R8-20251006","volume":"2","author":"Venkatesh Prajna","year":"2019","journal-title":"Peyman's Principles and Practice of Ophthalmology"},{"key":"R10-20251006","author":"Tille","year":"2014","journal-title":"Bailey and Scott's Diagnostic Microbiology"},{"key":"R11-20251006","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0002-9394(73)90008-1","article-title":"Spheroidal degeneration of cornea and conjunctiva;3 Incidences, classification, and etiology","volume":"76","author":"Fraunfelder","year":"1973","journal-title":"Am J Ophthalmol"},{"key":"R12-20251006","article-title":"Laboratory diagnosis of ocular infections","author":"Jones","year":"1994","journal-title":"Cumitech 13A. 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