{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:10:14Z","timestamp":1793214614930,"version":"4.5.0"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To study the clinical and microbiological profile of post-cataract surgery endophthalmitis and assess the utility of eubacterium and panfungal polymerase chain reaction (PCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      This was a case series of consecutive post-cataract surgery endophthalmitis patients treated at a tertiary care center in eastern India between January 2015 and December 2023. Data on clinical features, investigations, treatment, and outcomes were obtained from medical records. Aqueous or vitreous samples were first analyzed with various stains, including Gram\u2019s stain, 10% potassium hydroxide (KOH) mount, Giemsa stain, and Ziehl\u2013Nielsen stain. Further samples were inoculated into cultures. All samples were analyzed with polymerase chain reaction (PCR) to identity eubacterium, panfungal, and\n                      <jats:italic toggle=\"yes\">Propioniobacterium acnes<\/jats:italic>\n                      genomes. Favorable anatomical and functional outcomes were defined as clear media and attached retina and best-corrected visual acuity of 20\/200 or better, respectively. The main outcome measure was defined as anatomical and functional outcomes in culture-negative but PCR-positive and negative cases. Chi-square tests were performed for statistical analysis, and a\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      value of less than 0.05 was considered significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 132 patients were included. Culture was positive in 51 (38.6%) eyes. Gram-negative bacilli (27; 51.9%) were the predominant microorganisms, with\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      (11; 21.1%) being the most common isolate. Eubacterium or panfungal PCR were positive in 108 (81.8%) eyes. Among 81 culture-negative patients, 57 had positive PCR results (Group A), and 24 had negative PCR results (Group B). A favorable anatomical outcome was achieved in 51 (76.1%) patients in Group A and 10 (71.4%) in Group B (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.69). A favorable functional outcome was achieved in 50 (74.6%) patients in Group A and 9 (64.3%) patients in Group B (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.44).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>PCR positivity was higher than conventional culture. Eubacterium and panfungal PCR help in prompt decision-making in culture-negative endophthalmitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_2680_24","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T07:00:26Z","timestamp":1744873226000},"page":"870-874","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Clinico-microbiological profile of post-cataract surgery endophthalmitis and role of polymerase chain reaction in the management: A report from Eastern India"],"prefix":"10.4103","volume":"73","author":[{"given":"Pragati","family":"Paul","sequence":"first","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourav","family":"Padhi","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahana","family":"Sen","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aarzoo","family":"Juneja","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankeeta","family":"Baliarsingh","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eesh","family":"Nigam","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maitreyi","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surabhi","family":"Chattree","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalpita","family":"Das","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debmalya","family":"Das","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupak","family":"Roy","sequence":"additional","affiliation":[{"name":"Vitreo-Retina Services, Aditya Birla Sankara Nethralaya, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"R1-20260422","first-page":"249","article-title":"Cataract blindness challenges for 21st century","volume":"79","author":"Brain","year":"2001","journal-title":"Bull World Health Org"},{"key":"R2-20260422","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1038\/eye.2010.39","article-title":"Acute endophthalmitis after cataract surgery at a referral centre in Northern Taiwan: Review of the causative organisms, antibiotic susceptibility, and clinical features","volume":"24","author":"Cheng","year":"2010","journal-title":"Eye"},{"key":"R3-20260422","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.ophtha.2011.11.023","article-title":"Postcataract surgery endophthalmitis in the United States Analysis of the Complete 2003 to 2004 medicare database of cataract surgeries","volume":"119","author":"Keay","year":"2012","journal-title":"Ophthalmology"},{"key":"R4-20260422","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1136\/bjo.2008.147520","article-title":"Incidence and visual outcome of acute endophthalmitis after cataract surgery the experience of an eye department in Scotland","volume":"93","author":"Carrim","year":"2009","journal-title":"Br J Ophthalmol"},{"key":"R5-20260422","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1016\/j.ophtha.2005.05.020","article-title":"Postcataract endophthalmitis in South India. 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