{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:28:16Z","timestamp":1786649296894,"version":"build-2736575974"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To evaluate the correlation of quantitative real-time polymerase chain reaction (qRT-PCR) to the clinical characteristics of patients with viral retinitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Retrospective case series.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Aqueous or vitreous samples of 20 out of 35 eyes showed qRT-PCR positivity for virus etiology (57.14%). Cytomegalovirus (CMV) was most commonly identified in nine eyes (45%). The mean DNA copy number was 2,68,339.65 copies\/mL (range: 90\u20133205397). DNA copy number significantly correlated with the extent of clinical involvement (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.013); however, there was no correlation between DNA copy number and presenting visual acuity (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.31), macular involvement (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.675), optic nerve involvement (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.14), and development of retinal detachment (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.73). There was a significant correlation between the number of DNA copies and the timing of sampling (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.0005). Samples taken earlier in the course of the disease had higher viral copies than later ones.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>qRT-PCR is useful in confirming a viral etiology in over 50% of cases of suspected viral retinitis. It correlates well with the extent of clinical involvement and timing of sampling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_3000_21","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T07:18:21Z","timestamp":1654067901000},"page":"2090-2093","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Correlation of quantitative polymerase chain reaction with clinical characteristics of patients with viral retinitis"],"prefix":"10.4103","volume":"70","author":[{"given":"Sashwanthi","family":"Mohan","sequence":"first","affiliation":[{"name":"Department of Uvea, SankaraNethralaya, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Dhanurekha","sequence":"additional","affiliation":[{"name":"Sankara Nethralaya Referral Laboratory, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M K","family":"Janani","sequence":"additional","affiliation":[{"name":"Sankara Nethralaya Referral Laboratory, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotirmay","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Uvea, SankaraNethralaya, Medical Research Foundation, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"R1-20251004","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1001\/archopht.120.11.1534","article-title":"Real-time quantitative polymerase chain reaction diagnosis of infectious posterior uveitis","volume":"120","author":"Dworkin","year":"2002","journal-title":"Arch 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