{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:39:29Z","timestamp":1787841569807,"version":"build-2784847793"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To observe the spectroscopic profile of human lens in different age groups and varying grades of cataract and to use the data to arrive at differentiating molecular biology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design:<\/jats:title>\n                    <jats:p>An observational cross-sectional study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The study enrolled 30 patients (30 eyes) with a mean age of 59.6 years diagnosed with immature senile cataracts. The patients underwent small incision cataract surgery, and the harvested lens nuclei were examined under a Raman spectroscope for studying their molecular composition. The relative intensities of the peaks in the Raman spectra were evaluated and compared among different age groups and grades of cataract. A correlation of tyrosine doublet ratio with grade of cataract and age of the subject was calculated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result:<\/jats:title>\n                    <jats:p>\n                      Several Raman spectral peaks were observed in the range of 600 cm\n                      <jats:sup>-1<\/jats:sup>\n                      to 1800 cm\n                      <jats:sup>-1<\/jats:sup>\n                      with correspondence to tyrosine, phenylalanine, tryptophan, and amides I and III. A strong negative correlation between the grade of cataract and the ratio of tyrosine doublet was seen (r = -0.805). Also, a negative correlation between age and tyrosine ratio was seen (r = -0.62). The wavenumber\/spectral peak of tryptophan was observed only in one sample, and amides I and III were identified, but the intensity of the peak for amide II was very small or absent.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>It was observed that the buried conformation of tyrosine was predominant in cases with a higher age or grade of cataract. The buried conformation of tryptophan became less in the higher grades of cataract.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijo.ijo_3302_23","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T21:23:57Z","timestamp":1724448237000},"page":"1346-1351","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Effect of ageing and cataract formation on the Raman spectroscopic profile of human lens: An observational study"],"prefix":"10.4103","volume":"72","author":[{"given":"Ayasha","family":"Nishad","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, ABVIMS and Dr. RML Hospital, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, ABVIMS and Dr. RML Hospital, New Delhi, 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