{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:30:55Z","timestamp":1786649455681,"version":"build-2736575974"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,11]]},"abstract":"\n Purpose:<\/jats:title>\n \n To investigate the effects of hydroxybutyl chitosan (HBCS) with and without 5-fluorouracil (5-FU) during endoscopic endonasal dacryocystorhinostomy (Endo-DCR). In addition, the present study observed the impact of HBCS and 5-FU on the functions of the nasal mucosal cell population\n in vivo<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Patients were randomized into HBCS (group A), HBCS combined with 5-FU (group B), and gelatin sponge control group (group C). 16S rRNA high-throughput sequencing technology examined the conjunctival sac and nasal flora changes. In addition, CCK8, cell scratching, and flow cytometry were used to investigate the effects of HBCS and 5-FU on the nasal mucosal cell populations.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Subjects in groups A, B, and C had anastomotic areas of 21.83 \u00b1 12.69 mm\n 2<\/jats:sup>\n , 21.57 \u00b1 14.53 mm\n 2<\/jats:sup>\n , and 12.45 \u00b1 8.16 mm\n 2<\/jats:sup>\n , respectively (\n P<\/jats:italic>\n = 0.0359). Group A had less severe epiphora than the other two groups at 1-, 2-, and 12-week postoperative follow-up (\n P<\/jats:italic>\n < 0.05). Complications around the anastomosis in group A were the least severe of the three groups (\n P<\/jats:italic>\n = 0.0259). After surgery, the proportion of pathogenic bacteria in the conjunctival sac and nasal cavity was higher in groups A and B than in healthy adults. At the 2-week follow-up, the structure of nasal flora in group A was more similar to that of the healthy adults compared to group B.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Intraoperative use of HBCS at the anastomose improves the postoperative outcome of En-DCR. 5-FU cannot give better postoperative results in En-DCR and is detrimental to the normalization of the postoperative flora in patients with chronic dacryocystitis. At the cellular level, both HBCS and 5-FU inhibit the migration of nasal mucosal cell populations, and 5-FU inhibits proliferation but does not promote apoptosis.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijo.ijo_3375_23","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T03:02:07Z","timestamp":1726023727000},"page":"S838-S846","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["The impact of hydroxybutyl chitosan and 5-fluorouracil on the procedure of endoscopic endonasal dacryocystorhinostomy"],"prefix":"10.4103","volume":"72","author":[{"given":"Jia Qi","family":"Lin","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 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