{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:08:16Z","timestamp":1792001296222,"version":"4.4.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,5]]},"abstract":"\n CONTEXT:<\/jats:title>\n \n Enterococci<\/jats:italic>\n are known to cause life-threatening infections which are difficult to treat as the organism harbors innate resistance to many antibiotics and can amass resistance toward many others through plasmid-mediated genetic exchange.\n <\/jats:p>\n <\/jats:sec>\n \n AIMS:<\/jats:title>\n \n The study evaluates the drug susceptibility profile of various\n Enterococcus<\/jats:italic>\n species isolated from various patient specimens submitted for bacteriological analysis and check the incidence of\n aac(6\u2032) Ie-aph(2\u201d) Ia<\/jats:italic>\n gene.\n <\/jats:p>\n <\/jats:sec>\n \n SETTING AND DESIGN:<\/jats:title>\n \n This\n in vitro<\/jats:italic>\n cross-sectional study was executed at bacteriology laboratory of a 470 bedded hospital in New Delhi.\n <\/jats:p>\n <\/jats:sec>\n \n MATERIALS AND METHODS:<\/jats:title>\n \n Drug susceptibility testing was carried out on enterococcal isolates. High-level gentamicin-resistant (HLGR) isolates detected by micro broth dilution assay were then subjected to molecular detection of\n aac(6\u2032) Ie-aph(2\u201d) Ia<\/jats:italic>\n gene.\n <\/jats:p>\n <\/jats:sec>\n \n STATISTICAL ANALYSIS USED:<\/jats:title>\n The level of significance was established by Chi-square test.<\/jats:p>\n <\/jats:sec>\n \n RESULTS:<\/jats:title>\n \n Among the 182 enterococcal stains detected, 76.9% were\n Enterococcus faecalis<\/jats:italic>\n and 20.3% were\n Enterococcus faecium<\/jats:italic>\n . 12.08% strains were vancomycin resistant. 39% expressed resistance toward high-level gentamicin (HLG) and this finding was significantly higher in\n E. faecium<\/jats:italic>\n than\n E. faecalis<\/jats:italic>\n . HLGR strains expressed a higher degree of resistance to other drugs in contrast to non-HLGR isolates. In 67 out of 71 HLGR isolates the bifunctional gene was detected.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION:<\/jats:title>\n \n Considerable presence of HLG and vancomycin resistance in the clinical isolates is alarming and should be taken seriously. The study shows high dissemination of aac\n (6\u2032)-Ie-aph(2\u201d)-Ia<\/jats:italic>\n gene among\n Enterococci<\/jats:italic>\n isolated from the region.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijp.ijp_41_20","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T15:51:03Z","timestamp":1716479463000},"page":"171-176","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":9,"title":["High-level gentamicin resistance mediated by Aac(6\u2032)-Ie-aph(2\u201d)-Ia gene in Enterococcus species isolated from clinical samples in Northern India"],"prefix":"10.4103","volume":"54","author":[{"given":"Ayan Kumar","family":"Das","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mridu","family":"Dudeja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kohli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratima","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyamasree","family":"Nandy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R2-3-20240903","first-page":"e62921","article-title":"High-level gentamicin resistance and detection of aac(6') Ie-aph(2\u201d) Ia Gene in Enterococci isolated from Pediatric Hospital in Northwest of Iran","volume":"13","author":"Choukhachian","year":"2018","journal-title":"Arch Clin Infect 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