{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:38:56Z","timestamp":1786653536985,"version":"build-2736575974"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract:<\/jats:title>\n                  <jats:sec>\n                    <jats:title>OBJECTIVES:<\/jats:title>\n                    <jats:p>This study was set out to screen the cytotoxic activity of three extracts of two plants against three cancer cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>\n                      Cytotoxic activity of three extracts of these two plants (\n                      <jats:italic toggle=\"yes\">Haplophyllum tuberculatum<\/jats:italic>\n                      (H) and\n                      <jats:italic toggle=\"yes\">Sonchus oleraceus<\/jats:italic>\n                      L.(S)) was tested against three cell lines: HeLa uterus (H2), A431 skin (A2), and MCF breast (M2) using the MTT assay and the flow cytometric technique\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      The results revealed that similar yield percentages were obtained. Extract fractions from the first plant HF3 exerted high effects (&gt;75%) on the three cell lines. The aqueous extract of HF3 exhibited significant (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001) cell growth inhibition activities estimated as (inhibitory concentration 50 in m\/mL) of (0.73 \u00b1 0.08, 0.7566 \u00b1 0.12, and 0.65 \u00b1 0.04) on the HeLa, A431, and MCF7 cells, respectively, compared to standard doxorubicin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>The study concluded that similar yield percentages were obtained. Plant H exhibited higher activity, especially in its aqueous extract. Isolation, identification of the active (s) compounds, and determination of the mechanism of action were highly required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijp.ijp_621_24","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:03:09Z","timestamp":1777575789000},"page":"224-229","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Cytotoxic screening for three extracts of two plants using MTT assay and flow cytometric techniques"],"prefix":"10.4103","volume":"58","author":[{"given":"Aimun A. 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