{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:26:16Z","timestamp":1787772376916,"version":"build-2784847793"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:sec>\n                    <jats:title>OBJECTIVES:<\/jats:title>\n                    <jats:p>Isotretinoin (ISO) is a retinoic acid-derived molecule which is very efficient in the treatment of acne vulgaris (AV). Little is known regarding alterating affects of systemic ISO on immune system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SUBJECTS AND METHODS:<\/jats:title>\n                    <jats:p>Thirty-two patients with moderate and severe AV with indications for systemic ISO treatment were recruited. The evaluation of peripheral blood lymphocyte subsets was made on treatment initiation and at the end of the therapy by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      CD16\/56+ natural killer (NK) cell levels were higher in patients with severe acne compared to moderate acne (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.009). CD45RA+ (naive) cell levels decreased significantly after systemic ISO treatment; the mean percentage was 66.9 \u00b1 6.5 which decreased to 63.9 \u00b1 7.5 after treatment (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.013). CD19+ cell levels were also significantly increased; mean percentage (%) and absolute cell counts were 8.0 \u00b1 3.1 and 161.3 \u00b1 7.36 cells\/\u03bcl which increased to 10 \u00b1 2.7 and 227.7 \u00b1 108.7 cells\/\u03bcl, at the end of treatment, respectively (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.007,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.011).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>NK cell count may be a parameter related to acne severity. Systemic ISO may have an inhibitory effect on na\u00efve T cells. Finally, systemic ISO seems to stimulate mature B-cell proliferation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijp.ijp_695_21","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T15:47:53Z","timestamp":1716479273000},"page":"338-344","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of peripheral lymphocyte subsets in acne vulgaris patients before and after systemic isotretinoin treatment"],"prefix":"10.4103","volume":"54","author":[{"given":"Sibel Dogan","family":"Gunaydin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhan","family":"Tezcan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-5-20240903","doi-asserted-by":"crossref","first-page":"298","DOI":"10.4103\/0378-6323.199581","article-title":"Update on etiopathogenesis and treatment of Acne","volume":"83","author":"Bhat","year":"2017","journal-title":"Indian J Dermatol Venereol Leprol"},{"key":"R2-5-20240903","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/j.jaad.2015.12.037","article-title":"Guidelines of care for the management of acne vulgaris","volume":"74","author":"Zaenglein","year":"2016","journal-title":"J Am Acad Dermatol"},{"key":"R3-5-20240903","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.imbio.2014.02.010","article-title":"Reference ranges of lymphocyte subsets in healthy adults and adolescents with special mention of T cell maturation subsets in adults of South Florida","volume":"219","author":"Valiathan","year":"2014","journal-title":"Immunobiology"},{"key":"R4-5-20240903","first-page":"959","article-title":"Peripheral blood lymphocyte subsets in adolescents: A longitudinal analysis from the REACH project","volume":"9","author":"Rudy","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"R5-5-20240903","first-page":"739","article-title":"Lymphocyte subset reference ranges in healthy Israeli adults","volume":"18","author":"Shahal-Zimra","year":"2016","journal-title":"Isr Med Assoc J"},{"key":"R6-5-20240903","doi-asserted-by":"crossref","first-page":"443","DOI":"10.2217\/bmm.14.83","article-title":"Reference ranges of lymphocyte subsets in healthy Qatari adults","volume":"9","author":"Al-Thani","year":"2015","journal-title":"Biomark Med"},{"key":"R7-5-20240903","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1365-2133.1983.tb07081.x","article-title":"Lymphocyte subpopulations in patients with AV","volume":"109","author":"Holland","year":"1983","journal-title":"Br J Dermatol"},{"key":"R8-5-20240903","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2340\/000155557515","article-title":"Increased chemiluminescence of whole blood and normal T-lymphocyte subsets in severe nodular acne and acne fulminans","volume":"75","author":"Karvonen","year":"1995","journal-title":"Acta Derm Venereol"},{"key":"R9-5-20240903","first-page":"12","article-title":"Reduction of peripheral natural killer cells in patients with SAPHO syndrome","volume":"37","author":"Xu","year":"2019","journal-title":"Clin Exp Rheumatol"},{"key":"R10-5-20240903","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.3109\/09546634.2016.1145337","article-title":"How does our increased understanding of the role of inflammation and innate immunity in acne impact treatment approaches?","volume":"27","author":"Leyden","year":"2016","journal-title":"J Dermatolog Treat"},{"key":"R11-5-20240903","first-page":"20","article-title":"The expression of toll-like receptors in dermatological diseases and the therapeutic effect of current and newer topical toll-like receptor modulators","volume":"3","author":"Valins","year":"2010","journal-title":"J Clin Aesthet Dermatol"},{"key":"R12-5-20240903","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1038\/jid.2013.438","article-title":"IL-1b drives inflammatory responses to Propionibacterium acnes in vitro and in vivo","volume":"134","author":"Kistowska","year":"2014","journal-title":"J Invest Dermatol"},{"key":"R13-5-20240903","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/sji.12052","article-title":"Interaction of Mycobacterium tuberculosis cell wall components with the human natural killer cell receptors NKp44 and Toll-like receptor 2","volume":"77","author":"Esin","year":"2013","journal-title":"Scand J Immunol"},{"key":"R14-5-20240903","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1471-2350-11-17","article-title":"Toll-like receptor 2 gene polymorphisms, pulmonary tuberculosis, and natural killer cell counts","volume":"11","author":"Chen","year":"2010","journal-title":"BMC Med Genet"},{"key":"R15-5-20240903","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1365-2133.2004.05749.x","article-title":"Inflammation in acne scarring: A comparison of the responses in lesions from patients prone and not prone to scar","volume":"150","author":"Holland","year":"2004","journal-title":"Br J Dermatol"},{"key":"R16-5-20240903","first-page":"712","article-title":"Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes","volume":"143","author":"Tedder","year":"1989","journal-title":"A new member of the immunoglobulin superfamily. J Immunol"},{"key":"R17-5-20240903","doi-asserted-by":"crossref","first-page":"385","DOI":"10.3109\/10428199509059636","article-title":"CD19 antigen in leukemia and lymphoma diagnosis and immunotherapy","volume":"18","author":"Scheuermann","year":"1995","journal-title":"Leuk Lymphoma"},{"key":"R18-5-20240903","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/0192-0561(91)90060-K","article-title":"Differential effects of retinoids on pokeweed mitogen induced B-cell proliferation vs","volume":"13","author":"Dillehay","year":"1991","journal-title":"Immunoglobulin synthesis. Int J Immunopharmacol"},{"key":"R19-5-20240903","first-page":"477","article-title":"Long-term administration of 13-cis retinoic acid in common variable immunodeficiency: Circulating interleukin-6 levels, B-cell surface molecule display, and in vitro and in vivo B-cell antibody production","volume":"80","author":"Saxon","year":"1993","journal-title":"Immunology"},{"key":"R20-5-20240903","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/0091-6749(91)90176-O","article-title":"13-cis retinoic acid enhances in vivo B-lymphocyte differentiation in patients with common variable immunodeficiency","volume":"88","author":"Adelman","year":"1991","journal-title":"J Allergy Clin Immunol"},{"key":"R21-5-20240903","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1038\/sj.onc.1202089","article-title":"Retinoic acid-mediated growth arrest of EBV-immortalized B lymphocytes is associated with multiple changes in G1 regulatory proteins: P27KIP1 up-regulation is a relevant early event","volume":"17","author":"Zancai","year":"1998","journal-title":"Oncogene"},{"key":"R22-5-20240903","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1182\/blood-2006-09-046748","article-title":"Vitamin A potentiates CpG-mediated memory B-cell proliferation and differentiation: Involvement of early activation of p38MAPK","volume":"109","author":"Ertesvag","year":"2007","journal-title":"Blood"},{"key":"R23-5-20240903","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.cellimm.2012.09.003","article-title":"TLR9-signaling is required for turning retinoic acid into a potent stimulator of RP105 (CD180)-mediated proliferation and IgG synthesis in human memory B cells","volume":"279","author":"Eriksen","year":"2012","journal-title":"Cell Immunol"},{"key":"R24-5-20240903","doi-asserted-by":"crossref","first-page":"3624","DOI":"10.4049\/jimmunol.1300213","article-title":"Retinoic acid improves defective TLR9\/RP105-induced immune responses in common variable immunodeficiency-derived B cells","volume":"191","author":"Indrev\u00e6r","year":"2013","journal-title":"J Immunol"}],"container-title":["Indian Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ijp.ijp_695_21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:41:37Z","timestamp":1787769697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ijp.ijp_695_21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/ijp.ijp_695_21","relation":{},"ISSN":["0253-7613"],"issn-type":[{"value":"0253-7613","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9]]}}}