{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:28:12Z","timestamp":1786728492512,"version":"build-2736575974"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background\/Aim:<\/jats:title>\n \n Helicobacter pylori<\/jats:italic>\n (\n H. pylori<\/jats:italic>\n ) colonization affects the gastric microbiome, causing gastrointestinal (GI) diseases. Modern sequencing technology provides insights into GI microbe interaction with\n H. pylori<\/jats:italic>\n and their metabolic pathways in causing GI diseases. We aim to compare the gastric microbiota alteration due to\n H. pylori<\/jats:italic>\n infection in patients suffering from GI diseases.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Genomic DNA were isolated from gastric antrum tissue from 37\n H<\/jats:italic>\n .\n pylori<\/jats:italic>\n -infected patients diagnosed with GERD, duodenal ulcers, and gastritis. We conducted the genomic library preparation and sequencing of the amplified product using 16S rRNA NGS analysis. Using microbiome analyst tool diversity analysis, random forest analysis and ANOVA were conducted to find out the comparison of microbial abundance. We have also conducted functional pathway prediction analysis using PICRUSt.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Metagenomic analysis shows high bacterial diversity in\n H. pylori<\/jats:italic>\n -positive gastritis patients.\n Streptococcus infantis<\/jats:italic>\n and\n Neisseria subflava<\/jats:italic>\n were significantly higher in duodenal ulcer (DU) and gastritis groups.\n Acinetobacter lwoffii<\/jats:italic>\n and\n Helicobacter pullorum<\/jats:italic>\n were significantly high in the gastritis group only. The functional metabolic pathway analyses revealed that gastroesophageal reflux disease (GERD) samples were significantly enriched with the energy metabolism and xenobiotic biodegradation and metabolism pathways, whereas fructose-1,6-bisphosphatase III was found less in gastritis and DU groups.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n There is a difference in microbiota composition in different disease outcomes. We found positive association between microbial diversity and\n H. pylori<\/jats:italic>\n in gastritis group only, whereas negative association was found in DU and GERD groups. The functional metabolic pathway analysis revealed significant differences in various disease outcomes.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/ijpm.ijpm_1015_23","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T06:00:38Z","timestamp":1717740038000},"page":"61-68","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Alteration of gastric microbiota in Helicobacter pylori- infected individuals suffering from gastroesophageal reflux disease, duodenal ulcer, and gastritis"],"prefix":"10.4103","volume":"68","author":[{"given":"Prateek","family":"Sharma","sequence":"first","affiliation":[{"name":"Center for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarika","family":"Singh","sequence":"additional","affiliation":[{"name":"Center for Medical Biotechnology, 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