{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:32:01Z","timestamp":1787841121077,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aim:<\/jats:title>\n                    <jats:p>\n                      Ewing family of tumors (EFT) encompass a group of small blue round cell tumors, including Ewing sarcoma (ES) and\n                      <jats:italic toggle=\"yes\">EWSR1-<\/jats:italic>\n                      negative undifferentiated small round cell sarcoma. The distinction between the EFTs is essential from a clinical perspective due to prognostic and therapeutic differences and is substantiated by the advent of molecular testing in the modern era. In this study, we tried to characterize EFTs by using fluorescent\n                      <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                      hybridization (FISH) and reverse transcriptase polymerase chain reaction (RT-PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      A retrospective analysis of 47 cases diagnosed as Ewing sarcoma and undifferentiated small blue round cell tumors over a period of 3 years was done. Immunohistochemistry for CD99, NKX2.2, CCNB3, and DUX4 was performed.\n                      <jats:italic toggle=\"yes\">EWSR1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">FUS<\/jats:italic>\n                      gene rearrangement were performed with locus-specific break apart probes. Further characterization by RT-PCR analysis was attempted by using fusion primers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The mean age of presentation was 21.7 years. 34\/47 cases were of skeletal origin, while 13\/47 were of extraskeletal origin. Classical morphology was noted in 64% of the cases. All cases were CD99 positive, with variable patterns of expression. DUX4 and CCNB3 IHC were negative in all cases. FISH studies revealed\n                      <jats:italic toggle=\"yes\">EWSR1<\/jats:italic>\n                      rearrangement in 45\/47 cases, while the remaining 2\/47 showed\n                      <jats:italic toggle=\"yes\">FUS<\/jats:italic>\n                      rearrangement.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      In EFTs,\n                      <jats:italic toggle=\"yes\">EWSR1<\/jats:italic>\n                      gene rearrangement is most common, followed by\n                      <jats:italic toggle=\"yes\">FUS<\/jats:italic>\n                      gene rearrangement. CD99 immunohistochemistry with additional molecular analysis is essential for further classification of EFTs. A detailed diagnostic algorithm including histology, IHC, and molecular studies is imperative for accurate subcategorization of EFTs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijpm.ijpm_256_25","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T06:00:24Z","timestamp":1774936824000},"page":"40-47","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Morphological and molecular characterization of skeletal and extraskeletal Ewing family of tumors in a tertiary care center of eastern India"],"prefix":"10.4103","volume":"69","author":[{"given":"Mukund N.","family":"Sable","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JN","family":"Aparnna","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suvendu","family":"Purkait","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"R2-20260403","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.humpath.2024.01.007","article-title":"Updates on WHO classification for small round cell tumors:Ewing sarcoma vs. everything else","volume":"147","author":"Dehner","year":"2024","journal-title":"Hum 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