{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:34:38Z","timestamp":1786649678639,"version":"build-2736575974"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Calcium is an important substance to maintain the normal life activities of erythrocytes, and calcium overload has a damaging effect on erythrocytes. Multiple myeloma (MM) has a strong role in secreting microRNA (miRNA), but whether the miRNA produced by MM can affect the calcium regulation function of erythrocytes needs to be further studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The intracellular calcium content was detected by atomic absorption spectroscopy (AAS). The oxidative stress level of cells was assessed by the determination of ROS\/H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      \/GSH content of erythrocytes and the detection of antioxidant capacity. QRT-PCR and Western Blot were used to determine the expression of gene and protein. The binding relationship between miR-4261 and target genes was determined by a dual-luciferase gene reporter assay. Targeted drugs detect the effects of plasma membrane Ca\n                      <jats:sup>2+<\/jats:sup>\n                      -ATPase 4 (PMCA4) and calpain-1 on calcium regulation in erythrocytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      This study found that erythrocytes in MM had calcium overload and high levels of oxidative stress, which were associated with reduced plasma membrane Ca2+-ATPase4 (PMCA4) expression in erythrocytes. Expression of 14-3-3\u03b5 (Gene: YWHAE)\/calpastatin (Gene: CAST)\/glutathione peroxidase-1 (Gene: GPX1), which affected PMCA4 function, was also reduced. After database screening and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      studies, it was found that miR-4261 is the only miRNA in MM that can simultaneously downregulate the expression of 14-3-3\u03b5\/calpastatin\/GPX1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>MiR-4261 can target down-regulation of 14-3-3\u03b5\/calpastatin\/GPX1 to affect erythrocyte PMCA4, resulting in erythrocyte calcium overload and peroxidative damage. MiR-4261 may serve as one of the potential markers to measure erythrocyte damage in MM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijpm.ijpm_280_24","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:00:44Z","timestamp":1776261644000},"page":"245-252","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["MiR-4261 targeted YWHAE\/CAST\/GPX1 impairs the calcium regulatory function of PMCA4 in erythrocytes of myeloma"],"prefix":"10.4103","volume":"69","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Genetic Medicine, Shanxi Medical University, Taiyuan, Shanxi Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanxi Province Cancer Hospital, Taiyuan, Shanxi Province, 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