{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:30:29Z","timestamp":1786728629076,"version":"build-2736575974"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T00:00:00Z","timestamp":1725235200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Pityriasis versicolor (PV) is caused by a lipophilic fungus belonging to the genus\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      . Potassium hydroxide (KOH) mount is most frequently used for screening of cases and culture is the gold standard. KOH lacks sensitivity, and culture is time-consuming and technically demanding.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim, Settings, and Design:<\/jats:title>\n                    <jats:p>\n                      A cross-sectional study was conducted at a tertiary care teaching institution. We aimed to use multiplex-PCR for faster and accurate identification of\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      spp directly from skin scrapings of suspected cases of PV.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The study was conducted on suspected cases of PV over a period of 12 months. The clinical and demographic details were recorded. The skin scrapings were subjected to KOH mount and cultured on Sabouraud\u2019s dextrose agar with an olive oil overlay. Multiplex-PCR targeting 11\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      spp was performed on DNA extracted from skin scrapings.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 69 suspected cases of PV were studied. Most patients belonged to metro cities and worked in hot and humid climates. The mean duration of lesions was 18 months, and most had macular and patchy lesions. The sensitivity of KOH and culture was found to be 82.6% and 91.3%, respectively.\n                      <jats:italic toggle=\"yes\">M. globosa<\/jats:italic>\n                      (n = 60, 87%) and\n                      <jats:italic toggle=\"yes\">M. restricta<\/jats:italic>\n                      (n = 3, 4.3%) were isolated in culture. Multiplex PCR detected 85.5% of\n                      <jats:italic toggle=\"yes\">M. globosa<\/jats:italic>\n                      , 5.8% of\n                      <jats:italic toggle=\"yes\">M. restricta<\/jats:italic>\n                      , and 8.7% of mixed infection with\n                      <jats:italic toggle=\"yes\">M. globosa<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">M. restricta<\/jats:italic>\n                      . M-PCR detected\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      in all the samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      M-PCR could identify\n                      <jats:italic toggle=\"yes\">Malassezia<\/jats:italic>\n                      species directly from skin specimens, eliminating the need for culture. M-PCR was accurate, dependable, and exhibited a rapid turnaround time.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijpm.ijpm_85_24","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T09:26:13Z","timestamp":1725269173000},"page":"354-359","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Molecular identification of Malassezia from the skin scrapings of clinically suspected cases of pityriasis versicolor"],"prefix":"10.4103","volume":"68","author":[{"given":"Kanchankoti","family":"Kiran","sequence":"first","affiliation":[{"name":"Department of Microbiology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoorashetty Manohar","family":"Rudresh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoganand","family":"Raksha","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivanna","family":"Ragunatha","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Leprosy, ESIC MC PGIMSR and MH, Rajajinagar, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gupta","family":"Jyoti","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandya Rudramurthy","family":"Shivaprakash","sequence":"additional","affiliation":[{"name":"Department of Mycology, Postgraduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,9,2]]},"reference":[{"key":"R2-20250721","first-page":"18","article-title":"Isolation and speciation of Malassezia in patients clinically suspected of pityriasis versicolor","volume":"3","author":"Kumar","year":"2015","journal-title":"Int J Sci Stud"},{"key":"R3-20250721","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4103\/0377-4929.182027","article-title":"Clinicomycological profile of pityriasis versicolor in Assam","volume":"59","author":"Sharma","year":"2016","journal-title":"Indian J Pathol Microbiol"},{"key":"R4-20250721","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1080\/13693780701191373","article-title":"Update on the genus 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