{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:33:29Z","timestamp":1787762009309,"version":"build-2784847793"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>\n                      Trichomoniasis remains one of the most common sexually transmitted infections, which is curable. To prevent complications and transmission, prompt and correct diagnosis is essential to treat\n                      <jats:italic toggle=\"yes\">Trichomonas vaginalis<\/jats:italic>\n                      . The present study was done to evaluate polymerase chain reaction (PCR) with other conventional techniques for the diagnosis of\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      infection and determine the prevalence of\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      in women with vaginal discharge based on PCR assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Vaginal swabs were collected by the trained health-care professional using FLOQSwabs\u2122 (Copan, Italy) during routine pelvic examinations among 1974 symptomatic females. The wet microscopy, culture, and PCR were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The sensitivity of wet mount and culture in comparison to PCR was 60.87% and 56.52%, respectively. The kappa inter-rater agreement of\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      PCR showed substantial agreement with wet mount microscopy (\u03ba = 0.742) and culture (\u03ba = 0.707). The PCR detected an additional 17 cases that were missed by conventional techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion:<\/jats:title>\n                    <jats:p>\n                      The study highlights the importance of PCR for\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      screening among symptomatic females.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijstd.ijstd_21_22","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T03:01:40Z","timestamp":1685934100000},"page":"40-44","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Prevalence of Trichomonas vaginalis by polymerase chain reaction-based molecular method among symptomatic women from Northern 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