{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:33:33Z","timestamp":1787841213158,"version":"build-2784847793"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>\n                      The purpose of this\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      hair graft study was to better understand how different storage media affect the survival of hair follicles at different temperatures during a hair transplant procedure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      In this study, hair follicles (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 60) were harvested from 3 healthy male volunteers. Follicles were randomly assigned to the following groups: Group A: Storage media phosphate-buffered saline (PBS), platelet-rich plasma (PRP), platelet-rich fibrin (I-PRF), and Dulbecco\u2019s Modified Eagle Medium (DMEM). Group B: Storage media were placed at the following temperatures: 4\u00b0C, 26\u00b0C (room temperature), and 37\u00b0C (body temperature). The viability of hair follicles was checked using the explant culture method. Cell outgrowth was observed after incubation at 37\u00b0C in DMEM containing 10% FBS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Explant cultures of hair follicles stored at 4\u00b0C and 26\u00b0C did not show outgrowth of the cells after 7 days of culture. Explant cultures of hair follicles stored at 37\u00b0C did show outgrowth of the cells after 7 days of culture. Furthermore, these study results indicated that 10% DMEM preserves hair follicles more effectively than PBS, PRP, and I-PRF.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>According to the study\u2019s findings, freezing graft storage options might not be the best option. Instead of 4\u00b0C or room temperature 26\u00b0C, 37\u00b0C has been shown to improve hair graft survival. Furthermore, these study results indicated that 10% DMEM preserves hair follicles more effectively than PBS, PRP, and I-PRF. The study concluded that maintaining hair follicles in 10% DMEM at 37\u00b0C would prolong graft life and improve therapeutic outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijt.ijt_41_23","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:02:03Z","timestamp":1762250523000},"page":"121-127","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["An In vitro Study Comparing the Survival of Hair Follicles in Various Storage Media at Different Temperatures for Hair Transplant Procedure"],"prefix":"10.4103","volume":"17","author":[{"given":"Gaurav Madhukar","family":"Nerkar","sequence":"first","affiliation":[{"name":"Department of OMFS, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pushkar P.","family":"Waknis","sequence":"additional","affiliation":[{"name":"Department of OMFS, Dr. D. Y. Patil Dental College and Hospital, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash H.","family":"Kharat","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine Laboratory, Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin B.","family":"Yeole","sequence":"additional","affiliation":[{"name":"Swaroop Clinic, Nashik, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"R2-20251105","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2147\/CCID.S53980","article-title":"Hair restoration surgery: Challenges and solutions","volume":"8","author":"Rose","year":"2015","journal-title":"Clin Cosmet Investig Dermatol"},{"key":"R3-20251105","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4103\/0974-2077.69015","article-title":"Follicular unit extraction hair transplant","volume":"3","author":"Dua","year":"2010","journal-title":"J Cutan Aesthet Surg"},{"key":"R4-20251105","doi-asserted-by":"crossref","first-page":"69","DOI":"10.4103\/0974-2077.69014","article-title":"Review of factors affecting the growth and survival of follicular grafts","volume":"3","author":"Parsley","year":"2010","journal-title":"J Cutan Aesthet Surg"},{"key":"R5-20251105","first-page":"401","article-title":"How to improve graft survival in hair transplantation?","volume":"3","author":"Makharashvili","year":"2016","journal-title":"Glob Dermatol"},{"key":"R6-20251105","doi-asserted-by":"crossref","first-page":"17","DOI":"10.33589\/22.1.0017","article-title":"The optimal holding solution and temperature for hair follicle grafts","volume":"22","author":"Cole","year":"2012","journal-title":"Hair Transplant Forum Int"},{"key":"R7-20251105","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/jcbfm.1987.127","article-title":"Small differences in intraischemic brain temperature critically determine the extent of ischemic neuronal injury","volume":"7","author":"Busto","year":"1987","journal-title":"J Cereb Blood Flow Metab"},{"key":"R8-20251105","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1681\/ASN.2012080786","article-title":"Tolerance of the human kidney to isolated controlled ischemia","volume":"24","author":"Parekh","year":"2013","journal-title":"J Am Soc Nephrol"},{"key":"R11-20251105","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.fsc.2013.06.003","article-title":"Optimal graft growth","volume":"21","author":"Cooley","year":"2013","journal-title":"Facial Plast Surg Clin North Am"},{"key":"R12-20251105","doi-asserted-by":"crossref","first-page":"325","DOI":"10.33589\/13.3.0325","article-title":"New storage buffers for micrografts enhance graft survival and clinical outcome in hair restoration surgery","volume":"13","author":"Krugluger","year":"2003","journal-title":"Hair Transplant Forum Int"},{"key":"R13-20251105","doi-asserted-by":"crossref","first-page":"165","DOI":"10.33589\/31.5.165","article-title":"A study comparing survival of hair follicles stored cold and at room temperature","volume":"31","author":"Beehner","year":"2021","journal-title":"Hair Transplant Forum Int"},{"key":"R15-20251105","doi-asserted-by":"crossref","first-page":"352","DOI":"10.4103\/0378-6323.179089","article-title":"The forgotten hair transplantation experiment (1897) of Dr. Menahem Hodara (1869-1926)","volume":"82","author":"Tekiner","year":"2016","journal-title":"Indian J Dermatol Venereol Leprol"},{"key":"R16-20251105","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1346-8138.2009.00704.x","article-title":"Dr Shoji Okuda (1886-1962): The great pioneer of punch graft hair transplantation","volume":"36","author":"Inui","year":"2009","journal-title":"J Dermatol"},{"key":"R17-20251105","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1001\/jamadermatol.2017.2097","article-title":"Norman orentreich, the father of hair transplantation","volume":"153","author":"Choudhry","year":"2017","journal-title":"JAMA Dermatol"},{"key":"R18-20251105","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s12663-019-01245-6","article-title":"Follicular Unit Extraction (FUE) hair transplant: Curves ahead","volume":"18","author":"Sharma","year":"2019","journal-title":"J Maxillofac Oral Surg"},{"key":"R19-20251105","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.fsc.2020.01.006","article-title":"Follicular unit excision: Current practice and future developments","volume":"28","author":"Epstein","year":"2020","journal-title":"Facial Plast Surg Clin North Am"},{"issue":"Suppl","key":"R20-20251105","first-page":"S46","article-title":"Hair transplantation: Standard guidelines of care","volume":"74","author":"Patwardhan","year":"2008","journal-title":"Indian J Dermatol Venereol Leprol"},{"key":"R21-20251105","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.bjps.2004.05.020","article-title":"Is delayed micro-graft hair transplantation possible?Evaluation of viabilities of hair follicles preserved in two storage media","volume":"58","author":"Qian","year":"2005","journal-title":"Br J Plast Surg"},{"key":"R22-20251105","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1111\/j.1524-4725.1998.tb00012.x","article-title":"Power boosting the grafts in hair transplantation surgery. Evaluation of a new storage medium","volume":"24","author":"Raposio","year":"1998","journal-title":"Dermatol Surg"},{"key":"R23-20251105","doi-asserted-by":"crossref","first-page":"149","DOI":"10.5021\/ad.2002.14.3.149","article-title":"The effects of dehydration, preservation temperature, and time on the hair grafts","volume":"14","author":"Kim","year":"2002","journal-title":"Annals of Dermatology"},{"key":"R24-20251105","doi-asserted-by":"crossref","first-page":"e90","DOI":"10.1097\/GOX.0000000000000032","article-title":"The influence of preservation solution on the viability of grafts in hair transplantation surgery","volume":"1","author":"Gho","year":"2013","journal-title":"Plast Reconstr Surg Glob Open"},{"key":"R25-20251105","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00266-010-9549-z","article-title":"Oxidative stress in follicular units during hair transplantation surgery","volume":"35","author":"Cris\u00f3stomo","year":"2011","journal-title":"Aesthetic Plast Surg"},{"key":"R26-20251105","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1046\/j.1524-4725.1999.98287.x","article-title":"Effects of cooling micrografts in hair transplantation surgery","volume":"25","author":"Raposio","year":"1999","journal-title":"Dermatol Surg"},{"key":"R27-20251105","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1046\/j.1524-4725.1999.07319.x","article-title":"Viability of isolated single hair follicles preserved at 4 degrees C","volume":"25","author":"Kurata","year":"1999","journal-title":"Dermatol Surg"},{"key":"R28-20251105","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3109\/14764172.2015.1039037","article-title":"Hair follicles are viable after delayed FUE procedure","volume":"17","author":"Mohebbipour Laran","year":"2015","journal-title":"J Cosmet Laser Ther"},{"key":"R29-20251105","doi-asserted-by":"crossref","first-page":"e2875","DOI":"10.1097\/GOX.0000000000002875","article-title":"Effect of platelet rich plasma versus saline solution as a preservation solution for hair transplantation","volume":"8","author":"Abdelkader","year":"2020","journal-title":"Plast Reconstr Surg Glob Open"},{"key":"R30-20251105","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1097\/01.prs.0000239560.29172.33","article-title":"The role of platelet plasma growth factors in male pattern baldness surgery","volume":"118","author":"Uebel","year":"2006","journal-title":"Plast Reconstr Surg"},{"key":"R31-20251105","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1055\/s-0039-1696790","article-title":"A histological and clinical evaluation of plasma as a graft holding solution and its efficacy in terms of hair growth and graft survival","volume":"52","author":"Garg","year":"2019","journal-title":"Indian J Plast Surg"},{"key":"R32-20251105","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1097\/DSS.0000000000001895","article-title":"Impact of preservation solutions on the trichogenicity of hair micrografts ascertained by dermal papilla gene expression","volume":"45","author":"Castro","year":"2019","journal-title":"Dermatol Surg"},{"key":"R33-20251105","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1097\/DSS.0000000000001977","article-title":"A comparison of apoptotic activity for follicular unit extraction hair grafts stored in different holding solutions","volume":"46","author":"Rose","year":"2020","journal-title":"Dermatol Surg"},{"key":"R34-20251105","doi-asserted-by":"crossref","first-page":"e12334","DOI":"10.1111\/cpr.12334","article-title":"Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues","volume":"50","author":"Hendijani","year":"2017","journal-title":"Cell Prolif"}],"container-title":["International Journal of Trichology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/ijt.ijt_41_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:44:00Z","timestamp":1787838240000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/ijt.ijt_41_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/ijt.ijt_41_23","relation":{},"ISSN":["0974-7753","0974-9241"],"issn-type":[{"value":"0974-7753","type":"print"},{"value":"0974-9241","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2023-03-28","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-31","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}