{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:34:07Z","timestamp":1787762047861,"version":"build-2784847793"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Hair graft preservation is an important factor that influences graft survival in hair transplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To investigate the benefits of adding platelet-rich plasma (PRP), and the effect of different storage solutions and temperatures on hair follicle preservation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This randomized-controlled study included 10 androgenetic alopecia patients who underwent hair transplantation. Forty-five hair grafts were collected from each patient and then randomized to 8 different culture conditions for 7 days. Hair grafts were cultured in Williams\u2019 Medium E or Ringer\u2019s lactate solution (RLS) at either 4\u00b0C or 37\u00b0C, and with or without 10% PRP supplementation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      hair growth in Williams\u2019 Medium E was significantly greater than in RLS. The 37\u00b0C temperature condition was found to be significantly better than the 4\u00b0C condition. The growth of hair grafts cultured with PRP was not significance difference from those without PRP. However, immunofluorescence staining for cytokeratin 15 showed greater expression in hair graft cultured with PRP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>PRP may have a beneficial effect for preserving the viability of hair grafts. Williams\u2019 Medium E and 37\u00b0C temperature were found to be superior to RLS and 4\u00b0C relative to hair follicle growth in organ culture.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/ijt.ijt_65_22","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T16:14:26Z","timestamp":1690474466000},"page":"50-55","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Platelet-Rich Plasma and other Key Factors on Hair Follicle 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