{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:37:16Z","timestamp":1786649836615,"version":"build-2736575974"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>\n                    This study optimized supercritical CO\u2082 extraction (SCE) of\n                    <jats:italic toggle=\"yes\">Clinacanthus nutans<\/jats:italic>\n                    leaves, with or without 80:20 ethanol\u2013water co-solvent (CoS), and benchmarked it against ethanol maceration (MCE). MCE yielded 17.10% crude extract, while CoS SCE at 35 MPa\/60\u00b0C achieved 5.98%, but with higher enrichment of flavonoids (88 mg QE\/g, 2.1-fold above MCE) and retention of lipophilic phytosterols and terpenoids. MCE remained superior in total phenolic and saponin extraction. The selected SCE extract demonstrated dose- and time-dependent cytotoxicity against HeLa cells (IC\u2085\u2080 = 158 \u00b5g\/mL, 48 h), but limited selectivity over hTERT-HME1 cells (SI \u2248 1.2). These findings underscore CoS SCE as a greener, rapid method for selectively recovering moderately polar bioactives from\n                    <jats:italic toggle=\"yes\">C. nutans<\/jats:italic>\n                    , warranting bio-guided fractionation to enhance therapeutic specificity.\n                  <\/jats:p>","DOI":"10.4103\/japtr.japtr_101_25","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:01:28Z","timestamp":1760936488000},"page":"235-241","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Supercritical CO\u2082 extraction of Clinacanthus nutans: Enhanced bioactive recovery and cytotoxicity against cervical cancer cells"],"prefix":"10.4103","volume":"16","author":[{"given":"Lukkhana","family":"Benjawan","sequence":"first","affiliation":[{"name":"Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pakaporn","family":"Sathalalai","sequence":"additional","affiliation":[{"name":"Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somnuk","family":"Promdaeng","sequence":"additional","affiliation":[{"name":"Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriphan","family":"Sukkhung","sequence":"additional","affiliation":[{"name":"Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriwan","family":"Tipparak","sequence":"additional","affiliation":[{"name":"Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiriyaporn","family":"Sumsakul","sequence":"additional","affiliation":[{"name":"Expert Center of Innovative Herbal Products, Thailand Institute of Scientific and Technological Research, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanvisith","family":"Charoenying","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences, Pathumthani University, Pathum Thani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"R1-20260420","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jep.2017.05.007","article-title":"Ethnopharmacological uses, phytochemistry, biological activities, and therapeutic applications of Clinacanthus nutans (Burm. f.) 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Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/JAPTR.JAPTR_101_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:59:00Z","timestamp":1786647540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/JAPTR.JAPTR_101_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/japtr.japtr_101_25","relation":{},"ISSN":["2231-4040","0976-2094"],"issn-type":[{"value":"2231-4040","type":"print"},{"value":"0976-2094","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-04-28","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-13","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}