{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:31:04Z","timestamp":1786649464950,"version":"build-2736575974"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"\n The principal etiological agent responsible for dental caries is\n Streptococcus mutans<\/jats:italic>\n (S. mutans<\/jats:italic>\n ). The\n Moringa oleifera<\/jats:italic>\n (\n M. oleifera<\/jats:italic>\n ) possesses antioxidant and antibacterial properties that function through the response to oxidative stress, which affects bacterial cell metabolism. This research examined\n M. oleifera<\/jats:italic>\n impact on\n S. mutans<\/jats:italic>\n growth, toxicity, glucan-binding protein (GBP) expression, and nucleic acid structure. Methods included spectrophotometry for growth analysis, enzyme-linked immunosorbent assay for GBP quantification, the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay for cytotoxicity, Fourier transform infrared for nucleic acid changes, and docking simulation for ligand-receptor affinity. Results showed that\n M. oleifera<\/jats:italic>\n significantly inhibited\n S. mutans<\/jats:italic>\n growth at all concentrations over 24 and 48 h (optical density <0.1), comparable to <300 CFU\/mL. At 72 h, 6.25% and 3.125% concentrations were most effective, with chlorhexidine also showing stability at these times. A 3.125% concentration of\n M. oleifera<\/jats:italic>\n notably reduced GBP production to below 15% and caused cell toxicity. Furthermore, 25% and 3.125% concentrations significantly altered\n S. mutans<\/jats:italic>\n nucleic acids, and\n M. oleifera<\/jats:italic>\n showed high binding affinity to the GBP gene receptor. Thus,\n M. oleifera<\/jats:italic>\n can inhibit\n S. mutans<\/jats:italic>\n growth and GBP production, cause nucleic acid deformation, and strongly bind to the GBP receptor, highlighting its potential in dental caries prevention.\n <\/jats:p>","DOI":"10.4103\/japtr.japtr_442_23","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T06:12:44Z","timestamp":1721628764000},"page":"200-207","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Role of Moringa oleifera irrigation solution on the cell metabolism change of Streptococcus mutans"],"prefix":"10.4103","volume":"15","author":[{"given":"Cut","family":"Soraya","sequence":"first","affiliation":[{"name":"Department of Dentistry Conservative, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fitri Yunita","family":"Batubara","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Dentistry Faculty, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia L.","family":"Nasroen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Jenderal Achmad Yani, Cimahi, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhaini","family":"Jakfar","sequence":"additional","affiliation":[{"name":"Department of Dental Material, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basri A.","family":"Gani","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, 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