{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:23:13Z","timestamp":1787746993649,"version":"build-2784847793"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:p>\n                    <jats:italic toggle=\"yes\">Bridelia ovata<\/jats:italic>\n                    Decne. is a Thai traditional medicinal plant whose pharmacognostic standards, validated analytical methods, and anticancer mechanisms against triple-negative breast cancer (TNBC) remain unreported. The objective of this study was to establish a pharmacognostic monograph for\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">ovata<\/jats:italic>\n                    leaves, validate a high performance liquid chromatography (HPLC) method for epigallocatechin (EGC) quantification, profile the extract by liquid chromatography\/quadrupole time-of-flight mass spectrometry (LC\u2013QTOF-MS), and investigate signal transducer and activator of transcription 3 (STAT3)-mediated anti-proliferative mechanisms in MDA-MB-231 TNBC cells. One authenticated and 14 commercial\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">ovata<\/jats:italic>\n                    leaf samples were evaluated by macroscopic and microscopic examination, thin-layer chromatography fingerprinting, phytochemical screening, physicochemical analysis, validated HPLC, LC\u2013QTOF-MS profiling, network pharmacology, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and Western blotting. Key diagnostic features included paracytic stomata, lignified fibers, and calcium oxalate crystals. Most physicochemical parameters met World Health Organization general guidelines. The HPLC method showed excellent linearity (\n                    <jats:italic toggle=\"yes\">r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    &gt; 0.9995; 20\u2013500 \u03bcg\/mL); EGC content ranged from 0.20 to 11.05% w\/w. LC\u2013QTOF-MS tentatively identified 24 metabolites spanning phenolic acids, catechins, flavonoid glycosides, and terpenoids. Furthermore, network pharmacology identified STAT3 as the key hub target.\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">ovata<\/jats:italic>\n                    extract selectively inhibited MDA-MB-231 viability (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 413 \u00b1 23 \u03bcg\/mL vs. IC\n                    <jats:sub>50<\/jats:sub>\n                    &gt; 800 \u03bcg\/mL in MCF-10A), induced G2\/M arrest, and suppressed p-STAT3 (Tyr705) and cyclin D1 expression in a dose-dependent manner. These findings integrate pharmacognostic identification, physicochemical standardization, and mechanistic evidence of STAT3 pathway modulation, collectively supporting\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">ovata<\/jats:italic>\n                    as a phytopharmaceutical candidate for TNBC.\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">ovata<\/jats:italic>\n                    leaves demonstrate reproducible pharmacognostic identity, consistent EGC-based chemical quality markers, and STAT3-mediated antiproliferative activity, warranting further preclinical investigation.\n                  <\/jats:p>","DOI":"10.4103\/japtr.japtr_52_26","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:00:09Z","timestamp":1786546809000},"page":"296-303","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Pharmacognostic profiling of Bridelia ovata leaves and evaluation of signal transducer and activator of transcription 3-mediated antiproliferative effects in triple-negative breast cancer"],"prefix":"10.4103","volume":"17","author":[{"given":"Siti Nurfitriani","family":"Mandalika","sequence":"first","affiliation":[{"name":"Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasuni Gayathri","family":"Ileperuma","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Bangkok, Thailand"},{"name":"Center of Excellence in Natural Products for Ageing and Chronic Diseases, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krittapat","family":"Phairoh","sequence":"additional","affiliation":[{"name":"Department of Applied Thai Traditional Medicine, School of Integrative Medicine, Mae Fah Luang University, Chiang Rai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanundorn","family":"Thanusuwannasak","sequence":"additional","affiliation":[{"name":"CU-Drug and Health Products Innovation Promotion Center, Faculty of Pharmaceutical Sciences, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nonthaneth","family":"Nalinratana","sequence":"additional","affiliation":[{"name":"Center of Excellence in Natural Products for Ageing and Chronic Diseases, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worathat","family":"Thitikornpong","sequence":"additional","affiliation":[{"name":"Center of Excellence in Natural Products for Ageing and Chronic Diseases, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"},{"name":"Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"R1-20260826","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.jep.2012.02.019","article-title":"Medicinal plants used with Thai Traditional Medicine in modern healthcare services: A case study in Kabchoeng Hospital, Surin Province, Thailand","volume":"141","author":"Chotchoungchatchai","year":"2012","journal-title":"J Ethnopharmacol"},{"key":"R2-20260826","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jep.2009.05.019","article-title":"The genus Bridelia: A phytochemical and ethnopharmacological review","volume":"124","author":"Ngueyem","year":"2009","journal-title":"J Ethnopharmacol"},{"key":"R3-20260826","doi-asserted-by":"crossref","first-page":"e10366","DOI":"10.1016\/j.heliyon.2022.e10366","article-title":"Bridelia ferruginea Benth.; 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